High levels of 15-oxygenated steroids in circulation of patients with multiple sclerosis: Fact or fi ction?

I. Björkhem, A. Lövgren-Sandblom, F. Piehl, M. Khademi, H. Pettersson, V. Leoni, T. Olsson, U. Diczfalusy

Research output: Contribution to journalArticle

Abstract

15-Oxygenated cholesterol species such as 5 α-cholest-8(14)ene-3 β ,15 α -diol (15HC) and 3 β -hydroxy-5 α-cholest-8(14)- en-15-one (15KC) are commercially available synthetic products unlikely to occur in biological systems. Surprisingly, Farez et al. recently reported that these two steroids occur in human circulation at levels considerably higher than those of any other endogenous oxysterol [Farez, M. et al. 2009. Toll-like receptor 2 and poly(ADP-ribose) polymerase 1 promote central nervous system neuroinfl ammation in progressive EAE. Nat. Immunol. 10: 958-964]. The levels were reported to be increased in patients with multiple sclerosis in a progressive phase and the authors suggested that this could be utilized diagnostically. Based on extensive in vitro experiments exposing cells to the same high levels of 15HC as found in vivo (1000 ng/ml) the authors concluded that 15HC may be an important pathogenetic factor in multiple sclerosis. Using combined gas chromatography-mass spectrometry we fail to detect signifi-cant plasma levels of 15HC either in healthy controls or in patients with multiple sclerosis (levels <2 ng/ml). If 15KC is present in these plasma samples, the concentration of it must be

Original languageEnglish
Pages (from-to)170-174
Number of pages5
JournalJournal of Lipid Research
Volume52
Issue number1
DOIs
Publication statusPublished - Jan 2011

Keywords

  • 3 β -hydroxy-5 α -cholest-8(14)-en-15-one
  • 5 α -cholest-8(14)ene-3 β 15 α-diol
  • Autoxidation
  • Oxysterols

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Endocrinology

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  • Cite this

    Björkhem, I., Lövgren-Sandblom, A., Piehl, F., Khademi, M., Pettersson, H., Leoni, V., Olsson, T., & Diczfalusy, U. (2011). High levels of 15-oxygenated steroids in circulation of patients with multiple sclerosis: Fact or fi ction? Journal of Lipid Research, 52(1), 170-174. https://doi.org/10.1194/jlr.D011072