High levels of loss at the 17p telomere suggest the close proximity of a tumour suppressor

Grm White, M. Stack, M. Santibáñez-Koref, D. S. Liscia, T. Venesio, J. C. Wang, C. Helms, H. Donis-Keller, D. C. Betticher, H. J. Altermatt, P. R. Hoban, J. Heighway

Research output: Contribution to journalArticle

Abstract

High levels of loss of distal markers on 17p13.3 in breast cancer suggested the presence within the region of at least one tumour-suppressor gene. Here we describe the derivation of two biallelic polymorphisms from the 17p telomeric yeast artificial chromosome (YAC) TYAC98. Polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) and multiplex PCR analysis demonstrated that the high level of allelic imbalance observed in breast tumours represented loss of constitutional heterozygosity (LOH) and that this LOH extended to the telomere. Lung carcinoma (but not Wilms' tumour)-derived DNA again revealed a high level of loss of subtelomeric 17p sequences. Telomeric microsatellite polymorphisms from other chromosome arms did not show such elevated loss in either tumour type. This suggested that the 17p loss observed did not reflect a general telomeric instability and provided further evidence for the presence of a breast cancer tumour-suppressor gene in the distal region of 17p13.3.

Original languageEnglish
Pages (from-to)863-870
Number of pages8
JournalBritish Journal of Cancer
Volume74
Issue number6
Publication statusPublished - 1996

Fingerprint

Telomere
Tumor Suppressor Genes
Breast Neoplasms
Loss of Heterozygosity
Neoplasms
Allelic Imbalance
Yeast Artificial Chromosomes
Wilms Tumor
Multiplex Polymerase Chain Reaction
Restriction Fragment Length Polymorphisms
Microsatellite Repeats
Chromosomes
Carcinoma
Polymerase Chain Reaction
Lung
DNA

Keywords

  • Breast cancer
  • Chromosome 17
  • Loss of heterozygosity
  • Lung cancer

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

White, G., Stack, M., Santibáñez-Koref, M., Liscia, D. S., Venesio, T., Wang, J. C., ... Heighway, J. (1996). High levels of loss at the 17p telomere suggest the close proximity of a tumour suppressor. British Journal of Cancer, 74(6), 863-870.

High levels of loss at the 17p telomere suggest the close proximity of a tumour suppressor. / White, Grm; Stack, M.; Santibáñez-Koref, M.; Liscia, D. S.; Venesio, T.; Wang, J. C.; Helms, C.; Donis-Keller, H.; Betticher, D. C.; Altermatt, H. J.; Hoban, P. R.; Heighway, J.

In: British Journal of Cancer, Vol. 74, No. 6, 1996, p. 863-870.

Research output: Contribution to journalArticle

White, G, Stack, M, Santibáñez-Koref, M, Liscia, DS, Venesio, T, Wang, JC, Helms, C, Donis-Keller, H, Betticher, DC, Altermatt, HJ, Hoban, PR & Heighway, J 1996, 'High levels of loss at the 17p telomere suggest the close proximity of a tumour suppressor', British Journal of Cancer, vol. 74, no. 6, pp. 863-870.
White G, Stack M, Santibáñez-Koref M, Liscia DS, Venesio T, Wang JC et al. High levels of loss at the 17p telomere suggest the close proximity of a tumour suppressor. British Journal of Cancer. 1996;74(6):863-870.
White, Grm ; Stack, M. ; Santibáñez-Koref, M. ; Liscia, D. S. ; Venesio, T. ; Wang, J. C. ; Helms, C. ; Donis-Keller, H. ; Betticher, D. C. ; Altermatt, H. J. ; Hoban, P. R. ; Heighway, J. / High levels of loss at the 17p telomere suggest the close proximity of a tumour suppressor. In: British Journal of Cancer. 1996 ; Vol. 74, No. 6. pp. 863-870.
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AB - High levels of loss of distal markers on 17p13.3 in breast cancer suggested the presence within the region of at least one tumour-suppressor gene. Here we describe the derivation of two biallelic polymorphisms from the 17p telomeric yeast artificial chromosome (YAC) TYAC98. Polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) and multiplex PCR analysis demonstrated that the high level of allelic imbalance observed in breast tumours represented loss of constitutional heterozygosity (LOH) and that this LOH extended to the telomere. Lung carcinoma (but not Wilms' tumour)-derived DNA again revealed a high level of loss of subtelomeric 17p sequences. Telomeric microsatellite polymorphisms from other chromosome arms did not show such elevated loss in either tumour type. This suggested that the 17p loss observed did not reflect a general telomeric instability and provided further evidence for the presence of a breast cancer tumour-suppressor gene in the distal region of 17p13.3.

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