High magnesium inhibits human osteoblast differentiation in vitro

Marzia Leidi, Federica Dellera, Massimo Mariotti, Jeanette A M Maier

Research output: Contribution to journalArticle

Abstract

Several studies in humans indicate that both high and low concentrations of magnesium have harmful effects on bone metabolism and homeostasis. However, little is known about the effects of different concentrations of magnesium on bone cells. Considering that 1mM is the physiological concentration of extracellular magnesium for cultured cells, in our experimental model we exposed osteoblast like SaOS-2 cells and normal human osteoblasts to low (0.1mM) and high (5.0mM) concentrations of magnesium.We found that high concentrations of magnesium markedly inhibited the deposition of mineral matrix by SaOS-2 as well as the activity of alkaline phosphatase, a marker of osteoblast differentiation. We then evaluated the differentiation of normal human osteoblasts by measuring alkaline phosphatase activity and again found a marked inhibition by high concentrations of magnesium. Nitric oxide, which is known to play a role in bone formation, does not seem to be involved.We hypothesize that high levels of magnesium might alter the intracellular concentration of various cations - among which calcium - by competing for the same transporters. We conclude that high magnesium levels impair osteoblast activity and might therefore contribute to bone disease.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalMagnesium Research
Volume24
Issue number1
DOIs
Publication statusPublished - Mar 2011

Fingerprint

Osteoblasts
Magnesium
Bone
Alkaline Phosphatase
Bone and Bones
In Vitro Techniques
Bone Diseases
Differentiation Antigens
Metabolism
Osteogenesis
Minerals
Cations
Cultured Cells
Nitric Oxide
Homeostasis
Theoretical Models
Cells
Calcium

Keywords

  • Differentiation
  • Magnesium
  • Nitric oxide
  • Osteoblast

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

High magnesium inhibits human osteoblast differentiation in vitro. / Leidi, Marzia; Dellera, Federica; Mariotti, Massimo; Maier, Jeanette A M.

In: Magnesium Research, Vol. 24, No. 1, 03.2011, p. 1-6.

Research output: Contribution to journalArticle

Leidi, Marzia ; Dellera, Federica ; Mariotti, Massimo ; Maier, Jeanette A M. / High magnesium inhibits human osteoblast differentiation in vitro. In: Magnesium Research. 2011 ; Vol. 24, No. 1. pp. 1-6.
@article{ac805ffd4a724ed6b695e44ef007ce6d,
title = "High magnesium inhibits human osteoblast differentiation in vitro",
abstract = "Several studies in humans indicate that both high and low concentrations of magnesium have harmful effects on bone metabolism and homeostasis. However, little is known about the effects of different concentrations of magnesium on bone cells. Considering that 1mM is the physiological concentration of extracellular magnesium for cultured cells, in our experimental model we exposed osteoblast like SaOS-2 cells and normal human osteoblasts to low (0.1mM) and high (5.0mM) concentrations of magnesium.We found that high concentrations of magnesium markedly inhibited the deposition of mineral matrix by SaOS-2 as well as the activity of alkaline phosphatase, a marker of osteoblast differentiation. We then evaluated the differentiation of normal human osteoblasts by measuring alkaline phosphatase activity and again found a marked inhibition by high concentrations of magnesium. Nitric oxide, which is known to play a role in bone formation, does not seem to be involved.We hypothesize that high levels of magnesium might alter the intracellular concentration of various cations - among which calcium - by competing for the same transporters. We conclude that high magnesium levels impair osteoblast activity and might therefore contribute to bone disease.",
keywords = "Differentiation, Magnesium, Nitric oxide, Osteoblast",
author = "Marzia Leidi and Federica Dellera and Massimo Mariotti and Maier, {Jeanette A M}",
year = "2011",
month = "3",
doi = "10.1684/mrh.2011.0271",
language = "English",
volume = "24",
pages = "1--6",
journal = "Magnesium Research",
issn = "0953-1424",
publisher = "John Libbey Eurotext",
number = "1",

}

TY - JOUR

T1 - High magnesium inhibits human osteoblast differentiation in vitro

AU - Leidi, Marzia

AU - Dellera, Federica

AU - Mariotti, Massimo

AU - Maier, Jeanette A M

PY - 2011/3

Y1 - 2011/3

N2 - Several studies in humans indicate that both high and low concentrations of magnesium have harmful effects on bone metabolism and homeostasis. However, little is known about the effects of different concentrations of magnesium on bone cells. Considering that 1mM is the physiological concentration of extracellular magnesium for cultured cells, in our experimental model we exposed osteoblast like SaOS-2 cells and normal human osteoblasts to low (0.1mM) and high (5.0mM) concentrations of magnesium.We found that high concentrations of magnesium markedly inhibited the deposition of mineral matrix by SaOS-2 as well as the activity of alkaline phosphatase, a marker of osteoblast differentiation. We then evaluated the differentiation of normal human osteoblasts by measuring alkaline phosphatase activity and again found a marked inhibition by high concentrations of magnesium. Nitric oxide, which is known to play a role in bone formation, does not seem to be involved.We hypothesize that high levels of magnesium might alter the intracellular concentration of various cations - among which calcium - by competing for the same transporters. We conclude that high magnesium levels impair osteoblast activity and might therefore contribute to bone disease.

AB - Several studies in humans indicate that both high and low concentrations of magnesium have harmful effects on bone metabolism and homeostasis. However, little is known about the effects of different concentrations of magnesium on bone cells. Considering that 1mM is the physiological concentration of extracellular magnesium for cultured cells, in our experimental model we exposed osteoblast like SaOS-2 cells and normal human osteoblasts to low (0.1mM) and high (5.0mM) concentrations of magnesium.We found that high concentrations of magnesium markedly inhibited the deposition of mineral matrix by SaOS-2 as well as the activity of alkaline phosphatase, a marker of osteoblast differentiation. We then evaluated the differentiation of normal human osteoblasts by measuring alkaline phosphatase activity and again found a marked inhibition by high concentrations of magnesium. Nitric oxide, which is known to play a role in bone formation, does not seem to be involved.We hypothesize that high levels of magnesium might alter the intracellular concentration of various cations - among which calcium - by competing for the same transporters. We conclude that high magnesium levels impair osteoblast activity and might therefore contribute to bone disease.

KW - Differentiation

KW - Magnesium

KW - Nitric oxide

KW - Osteoblast

UR - http://www.scopus.com/inward/record.url?scp=79957465873&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79957465873&partnerID=8YFLogxK

U2 - 10.1684/mrh.2011.0271

DO - 10.1684/mrh.2011.0271

M3 - Article

C2 - 21421455

AN - SCOPUS:79957465873

VL - 24

SP - 1

EP - 6

JO - Magnesium Research

JF - Magnesium Research

SN - 0953-1424

IS - 1

ER -