High mobility group 1 (HMG1) protein in mouse preimplantation embryos

Fabio Spada, Adeline Brunet, Yvan Mercier, Jean Paul Renard, Marco E. Bianchi, Eric M. Thompson

Research output: Contribution to journalArticle

Abstract

High mobility group 1 protein (HMG1) has traditionally been considered a structural component of chromatin, possibly similar in function to histone H1. In fact, at the onset of Xenopus and Drosophila development, HMG1 appears to substitute for histone H1: HMG1 is abundant when histone H1 is absent after; the midblastula transition histone H1 largely replaces HMG1. We show that in early mouse embryos the expression patterns of HMG1 and histone H1 are not complementary. Instead, HMG1 content increases after zygotic genome activation at the same time as histone H1. HMG1 does not remain associated to mitotic chromosomes either in embryos or somatic cells. These results argue against a shared structural role for HMG1 and histone H1 in mammalian chromatin.

Original languageEnglish
Pages (from-to)57-66
Number of pages10
JournalMechanisms of Development
Volume76
Issue number1-2
DOIs
Publication statusPublished - Aug 1998

Keywords

  • Chromatin
  • Chromosomes
  • Histone H1
  • HMG-box
  • Luciferase
  • Metaphase
  • Microinjection
  • Midblastula transition
  • Mouse development
  • Nucleus
  • Oocyte
  • Transcription
  • Zygotic genome activation

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

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