High-mobility group box 1 release and redox regulation accompany regeneration and remodeling of skeletal muscle

Michela Vezzoli, Patrizia Castellani, Gianfranca Corna, Alessandra Castiglioni, Lidia Bosurgi, Antonella Monno, Silvia Brunelli, Angelo A. Manfredi, Anna Rubartelli, Patrizia Rovere-Querini

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

High-mobility group box 1 (HMGB1), a damage-associated molecular pattern (DAMP) molecules, favors tissue regeneration via recruitment and activation of leukocytes and stem cells. Here we demonstrate, in a model of acute sterile muscle injury, that regeneration is accompanied by active reactive oxygen species (ROS) production counterbalanced and overcome by the generation of antioxidant moieties. Mitochondria are initially responsible for ROS formation. However, they undergo rapid disruption with almost complete disappearance. Twenty-four hours after injury, we observed a strong induction of MURF1 and atrogin-1 ubiquitin ligases, key signals in activation of the proteasome system and induction of muscle atrophy. At later time points, ROS generation is maintained by nonmitochondrial sources. The antioxidant response occurs in both regenerating fibers and leukocytes that express high levels of free thiols and antioxidant enzymes, such as superoxide dismutase 1 (SOD1) and thioredoxin. HMGB1, a protein thiol, weakly expressed in healthy muscles, increases during regeneration in parallel with the antioxidant response in both fibers and leukocytes. A reduced environment may be important to maintain HMGB1 bioactivity. Indeed, oxidation abrogates both muscle stem cell migration in response to HMGB1 and their ability to differentiate into myofibers in vitro. We propose that the early antioxidant response in regenerating muscle limits HMGB1 oxidation, thus allowing successful muscle regeneration.

Original languageEnglish
Pages (from-to)2161-2174
Number of pages14
JournalAntioxidants and Redox Signaling
Volume15
Issue number8
DOIs
Publication statusPublished - Oct 15 2011

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Oxidation-Reduction
Muscle
Regeneration
Skeletal Muscle
Antioxidants
Reactive Oxygen Species
Muscles
Leukocytes
Sulfhydryl Compounds
Stem cells
Stem Cells
HMGB1 Protein
Chemical activation
Thioredoxins
Muscular Atrophy
Wounds and Injuries
Proteasome Endopeptidase Complex
Oxidation
Tissue regeneration
Ligases

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Physiology
  • Clinical Biochemistry

Cite this

High-mobility group box 1 release and redox regulation accompany regeneration and remodeling of skeletal muscle. / Vezzoli, Michela; Castellani, Patrizia; Corna, Gianfranca; Castiglioni, Alessandra; Bosurgi, Lidia; Monno, Antonella; Brunelli, Silvia; Manfredi, Angelo A.; Rubartelli, Anna; Rovere-Querini, Patrizia.

In: Antioxidants and Redox Signaling, Vol. 15, No. 8, 15.10.2011, p. 2161-2174.

Research output: Contribution to journalArticle

Vezzoli, Michela ; Castellani, Patrizia ; Corna, Gianfranca ; Castiglioni, Alessandra ; Bosurgi, Lidia ; Monno, Antonella ; Brunelli, Silvia ; Manfredi, Angelo A. ; Rubartelli, Anna ; Rovere-Querini, Patrizia. / High-mobility group box 1 release and redox regulation accompany regeneration and remodeling of skeletal muscle. In: Antioxidants and Redox Signaling. 2011 ; Vol. 15, No. 8. pp. 2161-2174.
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