Three chiral stationary phases, obtained by grafting silica gel with (-)-trans-1,2-cyclohexanediamine, were studied for the resolution of alpha -amino acids by ligand-exchange chromatography. The packings were prepared by bonding the chiral ligand to silica gel via different hydrocarbon spacers. Separation of the optical isomers was accomplished by eluents containing a constant concentration of copper(II) acetate (0. 05mM). The elution sequence of amino acids was found to be dependent on the grafting reaction selected to prepare the chiral packings.
|Number of pages||4|
|Journal||HRC & CC. Journal of high resolution chromatography & chromatography communications|
|Publication status||Published - May 1988|
ASJC Scopus subject areas