High-performance liquid chromatographic assay for the determination of Aloe Emodin in mouse plasma

M. Zaffaroni, C. Mucignat, T. Pecere, G. Zagotto, R. Frapolli, M. D'Incalci, M. Zucchetti

Research output: Contribution to journalArticlepeer-review

Abstract

An isocratic high-performance liquid chromatography (HPLC) method was developed and validated to determine Aloe Emodin (AE) in mouse plasma. The analysis required 0.3ml of plasma and involves extraction with dichloromethane. The HPLC separation was carried out on Symmetry Shield RP18, a mobile phase of methanol-water-acetic acid (65:35:0.2) and fluorescence detection at λ ex=410nm and λ em=510nm. The retention time of AE was 11.7min. The assay was linear from 10 to 1000ng/ml (r 2≥0.999), showed intra- and inter-day precision within 7.8 and 4.7%, and accuracy of 87.3-105.7%. Detection limit (LOD) and quantification limit (LOQ) were 4.5 and 5ng/ml, respectively. The method was applied to determine for the first time the pharmacokinetic of AE in mice.

Original languageEnglish
Pages (from-to)113-119
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume796
Issue number1
DOIs
Publication statusPublished - Oct 25 2003

Keywords

  • Aloe Emodin

ASJC Scopus subject areas

  • Biochemistry

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