TY - JOUR
T1 - High-performance liquid chromatographic assay for the determination of Aloe Emodin in mouse plasma
AU - Zaffaroni, M.
AU - Mucignat, C.
AU - Pecere, T.
AU - Zagotto, G.
AU - Frapolli, R.
AU - D'Incalci, M.
AU - Zucchetti, M.
PY - 2003/10/25
Y1 - 2003/10/25
N2 - An isocratic high-performance liquid chromatography (HPLC) method was developed and validated to determine Aloe Emodin (AE) in mouse plasma. The analysis required 0.3ml of plasma and involves extraction with dichloromethane. The HPLC separation was carried out on Symmetry Shield RP18, a mobile phase of methanol-water-acetic acid (65:35:0.2) and fluorescence detection at λ
ex=410nm and λ
em=510nm. The retention time of AE was 11.7min. The assay was linear from 10 to 1000ng/ml (r
2≥0.999), showed intra- and inter-day precision within 7.8 and 4.7%, and accuracy of 87.3-105.7%. Detection limit (LOD) and quantification limit (LOQ) were 4.5 and 5ng/ml, respectively. The method was applied to determine for the first time the pharmacokinetic of AE in mice.
AB - An isocratic high-performance liquid chromatography (HPLC) method was developed and validated to determine Aloe Emodin (AE) in mouse plasma. The analysis required 0.3ml of plasma and involves extraction with dichloromethane. The HPLC separation was carried out on Symmetry Shield RP18, a mobile phase of methanol-water-acetic acid (65:35:0.2) and fluorescence detection at λ
ex=410nm and λ
em=510nm. The retention time of AE was 11.7min. The assay was linear from 10 to 1000ng/ml (r
2≥0.999), showed intra- and inter-day precision within 7.8 and 4.7%, and accuracy of 87.3-105.7%. Detection limit (LOD) and quantification limit (LOQ) were 4.5 and 5ng/ml, respectively. The method was applied to determine for the first time the pharmacokinetic of AE in mice.
KW - Aloe Emodin
UR - http://www.scopus.com/inward/record.url?scp=1542608540&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1542608540&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2003.08.012
DO - 10.1016/j.jchromb.2003.08.012
M3 - Article
C2 - 14552822
AN - SCOPUS:1542608540
VL - 796
SP - 113
EP - 119
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 1
ER -