High-performance liquid chromatographic assay for the determination of the novel taxane derivative IDN5109 in mouse plasma

T. Colombo, R. Frapolli, E. Bombardelli, P. Morazzoni, A. Riva, M. D'Incalci, M. Zucchetti

Research output: Contribution to journalArticlepeer-review

Abstract

An HPLC assay was developed to determine the paclitaxel analogue 13-(N-tert.-butoxycarbonyl-β-isobutylisoserinyl)-14-hydroxybaccatin-1,14-carbonate (IDN5109) and its epimer in mouse plasma. The method involves solid-phase extraction on cyano cartridges (recovery >75%), HPLC separation on symmetry shield column, a mobile phase of NaH2PO4 (10 mM) pH 5.2, acetonitrile (47:53) and detection at 227 nm. Retention times of IDN5109, its epiform and internal standard were 15, 24 and 25.5 min, respectively. The assay was linear from 0.10 to 10 μg/ml (r2=0.999), with a C.V.

Original languageEnglish
Pages (from-to)135-141
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume736
Issue number1-2
DOIs
Publication statusPublished - Dec 24 1999

Keywords

  • IDN5109
  • Taxane

ASJC Scopus subject areas

  • Chemistry(all)

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