An HPLC assay was developed to determine the paclitaxel analogue 13-(N-tert.-butoxycarbonyl-β-isobutylisoserinyl)-14-hydroxybaccatin-1,14-carbonate (IDN5109) and its epimer in mouse plasma. The method involves solid-phase extraction on cyano cartridges (recovery >75%), HPLC separation on symmetry shield column, a mobile phase of NaH2PO4 (10 mM) pH 5.2, acetonitrile (47:53) and detection at 227 nm. Retention times of IDN5109, its epiform and internal standard were 15, 24 and 25.5 min, respectively. The assay was linear from 0.10 to 10 μg/ml (r2=0.999), with a C.V.
|Number of pages||7|
|Journal||Journal of Chromatography B: Biomedical Sciences and Applications|
|Publication status||Published - Dec 24 1999|
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