TY - JOUR
T1 - High-performance liquid chromatographic assay for the determination of the novel C-Seco-taxane derivative (IDN 5390) in mouse plasma
AU - Zaffaroni, M.
AU - Frapolli, R.
AU - Colombo, T.
AU - Fruscio, R.
AU - Bombardelli, E.
AU - Morazzoni, P.
AU - Riva, A.
AU - D'Incalci, M.
AU - Zucchetti, M.
PY - 2002/11/15
Y1 - 2002/11/15
N2 - A HPLC assay was developed to determine IDN 5390, a new paclitaxel analogue, in mouse plasma. The method involves solid-phase extraction from cyano cartridges (recovery >80%), HPLC separation on Symmetry C18 (4.6×150 mm), on isocratic mobile phase of water-acetonitrile-acetic acid (49:50:1) and detection at 227 nm. Retention times of IDN 5390 and IDN 5517 (internal standard, I.S.) were 9.1 and 10.5 min, respectively. The assay was linear from 0.05 to 5 μg/ml (r2≥0.995), showed intra- and inter-day precision within 1.0 and 6.2%, and accuracy of 94.7-106.8%. LOQ was 0.050 μg/ml. Using this method IDN 5390 pharmacokinetics was determined in mice.
AB - A HPLC assay was developed to determine IDN 5390, a new paclitaxel analogue, in mouse plasma. The method involves solid-phase extraction from cyano cartridges (recovery >80%), HPLC separation on Symmetry C18 (4.6×150 mm), on isocratic mobile phase of water-acetonitrile-acetic acid (49:50:1) and detection at 227 nm. Retention times of IDN 5390 and IDN 5517 (internal standard, I.S.) were 9.1 and 10.5 min, respectively. The assay was linear from 0.05 to 5 μg/ml (r2≥0.995), showed intra- and inter-day precision within 1.0 and 6.2%, and accuracy of 94.7-106.8%. LOQ was 0.050 μg/ml. Using this method IDN 5390 pharmacokinetics was determined in mice.
KW - C-Seco-taxane derivatives
KW - IDN 5390
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U2 - 10.1016/S1570-0232(02)00436-1
DO - 10.1016/S1570-0232(02)00436-1
M3 - Article
C2 - 12383484
AN - SCOPUS:0037111495
VL - 780
SP - 93
EP - 98
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 1
ER -