High-performance liquid chromatographic assay for the determination of the novel C-Seco-taxane derivative (IDN 5390) in mouse plasma

M. Zaffaroni; R. Frapolli; T. Colombo; R. Fruscio; E. Bombardelli; P. Morazzoni; A. Riva; M. D'Incalci; M. Zucchetti

doi:10.1016/S1570-0232(02)00436-1

High-performance liquid chromatographic assay for the determination of the novel C-Seco-taxane derivative (IDN 5390) in mouse plasma

M. Zaffaroni, R. Frapolli, T. Colombo, R. Fruscio, E. Bombardelli, P. Morazzoni, A. Riva, M. D'Incalci, M. Zucchetti

Istituto di Ricerche Farmacologiche "Mario Negri"

Research output: Contribution to journal › Article › peer-review

Abstract

A HPLC assay was developed to determine IDN 5390, a new paclitaxel analogue, in mouse plasma. The method involves solid-phase extraction from cyano cartridges (recovery >80%), HPLC separation on Symmetry C₁₈ (4.6×150 mm), on isocratic mobile phase of water-acetonitrile-acetic acid (49:50:1) and detection at 227 nm. Retention times of IDN 5390 and IDN 5517 (internal standard, I.S.) were 9.1 and 10.5 min, respectively. The assay was linear from 0.05 to 5 μg/ml (r²≥0.995), showed intra- and inter-day precision within 1.0 and 6.2%, and accuracy of 94.7-106.8%. LOQ was 0.050 μg/ml. Using this method IDN 5390 pharmacokinetics was determined in mice.

Original language	English
Pages (from-to)	93-98
Number of pages	6
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	780
Issue number	1
DOIs	https://doi.org/10.1016/S1570-0232(02)00436-1
Publication status	Published - Nov 15 2002

Keywords

C-Seco-taxane derivatives
IDN 5390

ASJC Scopus subject areas

Biochemistry

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10.1016/S1570-0232(02)00436-1

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Zaffaroni, M., Frapolli, R., Colombo, T., Fruscio, R., Bombardelli, E., Morazzoni, P., Riva, A., D'Incalci, M., & Zucchetti, M. (2002). High-performance liquid chromatographic assay for the determination of the novel C-Seco-taxane derivative (IDN 5390) in mouse plasma. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 780(1), 93-98. https://doi.org/10.1016/S1570-0232(02)00436-1

High-performance liquid chromatographic assay for the determination of the novel C-Seco-taxane derivative (IDN 5390) in mouse plasma. / Zaffaroni, M.; Frapolli, R.; Colombo, T.; Fruscio, R.; Bombardelli, E.; Morazzoni, P.; Riva, A.; D'Incalci, M.; Zucchetti, M.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 780, No. 1, 15.11.2002, p. 93-98.

Research output: Contribution to journal › Article › peer-review

Zaffaroni, M, Frapolli, R, Colombo, T, Fruscio, R, Bombardelli, E, Morazzoni, P, Riva, A, D'Incalci, M & Zucchetti, M 2002, 'High-performance liquid chromatographic assay for the determination of the novel C-Seco-taxane derivative (IDN 5390) in mouse plasma', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 780, no. 1, pp. 93-98. https://doi.org/10.1016/S1570-0232(02)00436-1

Zaffaroni M, Frapolli R, Colombo T, Fruscio R, Bombardelli E, Morazzoni P et al. High-performance liquid chromatographic assay for the determination of the novel C-Seco-taxane derivative (IDN 5390) in mouse plasma. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2002 Nov 15;780(1):93-98. https://doi.org/10.1016/S1570-0232(02)00436-1

Zaffaroni, M. ; Frapolli, R. ; Colombo, T. ; Fruscio, R. ; Bombardelli, E. ; Morazzoni, P. ; Riva, A. ; D'Incalci, M. ; Zucchetti, M. / High-performance liquid chromatographic assay for the determination of the novel C-Seco-taxane derivative (IDN 5390) in mouse plasma. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2002 ; Vol. 780, No. 1. pp. 93-98.

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abstract = "A HPLC assay was developed to determine IDN 5390, a new paclitaxel analogue, in mouse plasma. The method involves solid-phase extraction from cyano cartridges (recovery >80%), HPLC separation on Symmetry C18 (4.6×150 mm), on isocratic mobile phase of water-acetonitrile-acetic acid (49:50:1) and detection at 227 nm. Retention times of IDN 5390 and IDN 5517 (internal standard, I.S.) were 9.1 and 10.5 min, respectively. The assay was linear from 0.05 to 5 μg/ml (r2≥0.995), showed intra- and inter-day precision within 1.0 and 6.2%, and accuracy of 94.7-106.8%. LOQ was 0.050 μg/ml. Using this method IDN 5390 pharmacokinetics was determined in mice.",

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AU - Colombo, T.

AU - Fruscio, R.

AU - Bombardelli, E.

AU - Morazzoni, P.

AU - Riva, A.

AU - D'Incalci, M.

AU - Zucchetti, M.

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High-performance liquid chromatographic assay for the determination of the novel C-Seco-taxane derivative (IDN 5390) in mouse plasma

Abstract

Keywords

ASJC Scopus subject areas

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