High-performance liquid chromatographic assay for the determination of the novel C-Seco-taxane derivative (IDN 5390) in mouse plasma

M. Zaffaroni, R. Frapolli, T. Colombo, R. Fruscio, E. Bombardelli, P. Morazzoni, A. Riva, M. D'Incalci, M. Zucchetti

Research output: Contribution to journalArticlepeer-review

Abstract

A HPLC assay was developed to determine IDN 5390, a new paclitaxel analogue, in mouse plasma. The method involves solid-phase extraction from cyano cartridges (recovery >80%), HPLC separation on Symmetry C18 (4.6×150 mm), on isocratic mobile phase of water-acetonitrile-acetic acid (49:50:1) and detection at 227 nm. Retention times of IDN 5390 and IDN 5517 (internal standard, I.S.) were 9.1 and 10.5 min, respectively. The assay was linear from 0.05 to 5 μg/ml (r2≥0.995), showed intra- and inter-day precision within 1.0 and 6.2%, and accuracy of 94.7-106.8%. LOQ was 0.050 μg/ml. Using this method IDN 5390 pharmacokinetics was determined in mice.

Original languageEnglish
Pages (from-to)93-98
Number of pages6
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume780
Issue number1
DOIs
Publication statusPublished - Nov 15 2002

Keywords

  • C-Seco-taxane derivatives
  • IDN 5390

ASJC Scopus subject areas

  • Biochemistry

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