High-performance liquid chromatographic determination of 5-bromodeoxyuridine in human plasma

Paolo G. Montaldo, Maurizio D'Incalci

Research output: Contribution to journalArticlepeer-review

Abstract

A new method is described for the determination of submicromolar concentrations of 5-bromodeoxyuridine in human plasma. Sample pretreatment involves cold methanol deproteinization, freezing-thawing and lyophilization. The sample is then analysed by reversed-phase high-performance liquid chromatography. This method is very reproducible and has a detection limit of 0.1 μg/ml (0.32·10-6 M). Comparison with other procedures indicates that the method is advantageous as regards sensitivity and specificity and can be readily applied in clinical pharmacological investigations.

Original languageEnglish
Pages (from-to)129-138
Number of pages10
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume491
Issue numberC
DOIs
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

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