High-performance liquid chromatographic determination of 5-bromodeoxyuridine in human plasma

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4 Citations (Scopus)

Abstract

A new method is described for the determination of submicromolar concentrations of 5-bromodeoxyuridine in human plasma. Sample pretreatment involves cold methanol deproteinization, freezing-thawing and lyophilization. The sample is then analysed by reversed-phase high-performance liquid chromatography. This method is very reproducible and has a detection limit of 0.1 μg/ml (0.32·10-6 M). Comparison with other procedures indicates that the method is advantageous as regards sensitivity and specificity and can be readily applied in clinical pharmacological investigations.

Original languageEnglish
Pages (from-to)129-138
Number of pages10
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume491
Issue numberC
DOIs
Publication statusPublished - 1989

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Plasma (human)
Thawing
High performance liquid chromatography
Bromodeoxyuridine
Freezing
Methanol
Liquids
Freeze Drying
Reverse-Phase Chromatography
Limit of Detection
High Pressure Liquid Chromatography
Pharmacology
Sensitivity and Specificity

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

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title = "High-performance liquid chromatographic determination of 5-bromodeoxyuridine in human plasma",
abstract = "A new method is described for the determination of submicromolar concentrations of 5-bromodeoxyuridine in human plasma. Sample pretreatment involves cold methanol deproteinization, freezing-thawing and lyophilization. The sample is then analysed by reversed-phase high-performance liquid chromatography. This method is very reproducible and has a detection limit of 0.1 μg/ml (0.32·10-6 M). Comparison with other procedures indicates that the method is advantageous as regards sensitivity and specificity and can be readily applied in clinical pharmacological investigations.",
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T1 - High-performance liquid chromatographic determination of 5-bromodeoxyuridine in human plasma

AU - Montaldo, Paolo G.

AU - D'Incalci, Maurizio

PY - 1989

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N2 - A new method is described for the determination of submicromolar concentrations of 5-bromodeoxyuridine in human plasma. Sample pretreatment involves cold methanol deproteinization, freezing-thawing and lyophilization. The sample is then analysed by reversed-phase high-performance liquid chromatography. This method is very reproducible and has a detection limit of 0.1 μg/ml (0.32·10-6 M). Comparison with other procedures indicates that the method is advantageous as regards sensitivity and specificity and can be readily applied in clinical pharmacological investigations.

AB - A new method is described for the determination of submicromolar concentrations of 5-bromodeoxyuridine in human plasma. Sample pretreatment involves cold methanol deproteinization, freezing-thawing and lyophilization. The sample is then analysed by reversed-phase high-performance liquid chromatography. This method is very reproducible and has a detection limit of 0.1 μg/ml (0.32·10-6 M). Comparison with other procedures indicates that the method is advantageous as regards sensitivity and specificity and can be readily applied in clinical pharmacological investigations.

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JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

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IS - C

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