High-performance liquid chromatographic determination of desmosine and isodesmosine after phenylisothiocyanate derivatization

M. Salomoni; M. Muda; E. Zuccato; E. Mussini

doi:10.1016/0378-4347(91)80496-Y

High-performance liquid chromatographic determination of desmosine and isodesmosine after phenylisothiocyanate derivatization

M. Salomoni, M. Muda, E. Zuccato, E. Mussini

Istituto di Ricerche Farmacologiche "Mario Negri"

Research output: Contribution to journal › Article › peer-review

Abstract

A new sensitive and selective high-performance liquid chromatographic method for the analysis of desmosine and isodesmosine in human and rat tissues is described. This method requires a purification step with column chromatography, followed by precolumn derivatization with phenylisothiocyanate. The reaction products are then separated by isocratic chromatography on a C₁₈ column and quantitated by ultraviolet detection at 254 nm. The recovery of standards of both compounds added to tissue samples and analysed by this method is usually greater than 90%, and the absolute detection limit is 0.5 ng for both compounds. The method is sensitive enough to measure both substances in tissue fragments of 30 mg of wet mass, which means that it can be used to study elastin in small human biopsies.

Original language	English
Pages (from-to)	312-316
Number of pages	5
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	572
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-4347(91)80496-Y
Publication status	Published - Dec 6 1991

ASJC Scopus subject areas

Chemistry(all)
Clinical Biochemistry
Molecular Medicine

Access to Document

10.1016/0378-4347(91)80496-Y

Fingerprint Dive into the research topics of 'High-performance liquid chromatographic determination of desmosine and isodesmosine after phenylisothiocyanate derivatization'. Together they form a unique fingerprint.

Cite this

@article{08c2bca1e0364c2e82ed12fc41b432b8,

title = "High-performance liquid chromatographic determination of desmosine and isodesmosine after phenylisothiocyanate derivatization",

abstract = "A new sensitive and selective high-performance liquid chromatographic method for the analysis of desmosine and isodesmosine in human and rat tissues is described. This method requires a purification step with column chromatography, followed by precolumn derivatization with phenylisothiocyanate. The reaction products are then separated by isocratic chromatography on a C18 column and quantitated by ultraviolet detection at 254 nm. The recovery of standards of both compounds added to tissue samples and analysed by this method is usually greater than 90%, and the absolute detection limit is 0.5 ng for both compounds. The method is sensitive enough to measure both substances in tissue fragments of 30 mg of wet mass, which means that it can be used to study elastin in small human biopsies.",

author = "M. Salomoni and M. Muda and E. Zuccato and E. Mussini",

year = "1991",

month = dec,

day = "6",

doi = "10.1016/0378-4347(91)80496-Y",

language = "English",

volume = "572",

pages = "312--316",

journal = "Journal of Chromatography B: Biomedical Sciences and Applications",

issn = "1387-2273",

publisher = "Elsevier BV",

number = "1-2",

}

TY - JOUR

T1 - High-performance liquid chromatographic determination of desmosine and isodesmosine after phenylisothiocyanate derivatization

AU - Salomoni, M.

AU - Muda, M.

AU - Zuccato, E.

AU - Mussini, E.

PY - 1991/12/6

Y1 - 1991/12/6

N2 - A new sensitive and selective high-performance liquid chromatographic method for the analysis of desmosine and isodesmosine in human and rat tissues is described. This method requires a purification step with column chromatography, followed by precolumn derivatization with phenylisothiocyanate. The reaction products are then separated by isocratic chromatography on a C18 column and quantitated by ultraviolet detection at 254 nm. The recovery of standards of both compounds added to tissue samples and analysed by this method is usually greater than 90%, and the absolute detection limit is 0.5 ng for both compounds. The method is sensitive enough to measure both substances in tissue fragments of 30 mg of wet mass, which means that it can be used to study elastin in small human biopsies.

AB - A new sensitive and selective high-performance liquid chromatographic method for the analysis of desmosine and isodesmosine in human and rat tissues is described. This method requires a purification step with column chromatography, followed by precolumn derivatization with phenylisothiocyanate. The reaction products are then separated by isocratic chromatography on a C18 column and quantitated by ultraviolet detection at 254 nm. The recovery of standards of both compounds added to tissue samples and analysed by this method is usually greater than 90%, and the absolute detection limit is 0.5 ng for both compounds. The method is sensitive enough to measure both substances in tissue fragments of 30 mg of wet mass, which means that it can be used to study elastin in small human biopsies.

UR - http://www.scopus.com/inward/record.url?scp=0026351544&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026351544&partnerID=8YFLogxK

U2 - 10.1016/0378-4347(91)80496-Y

DO - 10.1016/0378-4347(91)80496-Y

M3 - Article

C2 - 1818066

AN - SCOPUS:0026351544

VL - 572

SP - 312

EP - 316

JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

SN - 1387-2273

IS - 1-2

ER -