High-performance liquid chromatographic determination of desmosine and isodesmosine after phenylisothiocyanate derivatization

M. Salomoni, M. Muda, E. Zuccato, E. Mussini

Research output: Contribution to journalArticlepeer-review

Abstract

A new sensitive and selective high-performance liquid chromatographic method for the analysis of desmosine and isodesmosine in human and rat tissues is described. This method requires a purification step with column chromatography, followed by precolumn derivatization with phenylisothiocyanate. The reaction products are then separated by isocratic chromatography on a C18 column and quantitated by ultraviolet detection at 254 nm. The recovery of standards of both compounds added to tissue samples and analysed by this method is usually greater than 90%, and the absolute detection limit is 0.5 ng for both compounds. The method is sensitive enough to measure both substances in tissue fragments of 30 mg of wet mass, which means that it can be used to study elastin in small human biopsies.

Original languageEnglish
Pages (from-to)312-316
Number of pages5
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume572
Issue number1-2
DOIs
Publication statusPublished - Dec 6 1991

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

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