Abstract
A new sensitive and selective high-performance liquid chromatographic method for the analysis of desmosine and isodesmosine in human and rat tissues is described. This method requires a purification step with column chromatography, followed by precolumn derivatization with phenylisothiocyanate. The reaction products are then separated by isocratic chromatography on a C18 column and quantitated by ultraviolet detection at 254 nm. The recovery of standards of both compounds added to tissue samples and analysed by this method is usually greater than 90%, and the absolute detection limit is 0.5 ng for both compounds. The method is sensitive enough to measure both substances in tissue fragments of 30 mg of wet mass, which means that it can be used to study elastin in small human biopsies.
| Original language | English |
|---|---|
| Pages (from-to) | 312-316 |
| Number of pages | 5 |
| Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
| Volume | 572 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Dec 6 1991 |
Fingerprint
ASJC Scopus subject areas
- Chemistry(all)
- Clinical Biochemistry
- Molecular Medicine
Cite this
High-performance liquid chromatographic determination of desmosine and isodesmosine after phenylisothiocyanate derivatization. / Salomoni, M.; Muda, M.; Zuccato, E.; Mussini, E.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 572, No. 1-2, 06.12.1991, p. 312-316.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High-performance liquid chromatographic determination of desmosine and isodesmosine after phenylisothiocyanate derivatization
AU - Salomoni, M.
AU - Muda, M.
AU - Zuccato, E.
AU - Mussini, E.
PY - 1991/12/6
Y1 - 1991/12/6
N2 - A new sensitive and selective high-performance liquid chromatographic method for the analysis of desmosine and isodesmosine in human and rat tissues is described. This method requires a purification step with column chromatography, followed by precolumn derivatization with phenylisothiocyanate. The reaction products are then separated by isocratic chromatography on a C18 column and quantitated by ultraviolet detection at 254 nm. The recovery of standards of both compounds added to tissue samples and analysed by this method is usually greater than 90%, and the absolute detection limit is 0.5 ng for both compounds. The method is sensitive enough to measure both substances in tissue fragments of 30 mg of wet mass, which means that it can be used to study elastin in small human biopsies.
AB - A new sensitive and selective high-performance liquid chromatographic method for the analysis of desmosine and isodesmosine in human and rat tissues is described. This method requires a purification step with column chromatography, followed by precolumn derivatization with phenylisothiocyanate. The reaction products are then separated by isocratic chromatography on a C18 column and quantitated by ultraviolet detection at 254 nm. The recovery of standards of both compounds added to tissue samples and analysed by this method is usually greater than 90%, and the absolute detection limit is 0.5 ng for both compounds. The method is sensitive enough to measure both substances in tissue fragments of 30 mg of wet mass, which means that it can be used to study elastin in small human biopsies.
UR - http://www.scopus.com/inward/record.url?scp=0026351544&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026351544&partnerID=8YFLogxK
U2 - 10.1016/0378-4347(91)80496-Y
DO - 10.1016/0378-4347(91)80496-Y
M3 - Article
C2 - 1818066
AN - SCOPUS:0026351544
VL - 572
SP - 312
EP - 316
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 1387-2273
IS - 1-2
ER -