High-performance liquid chromatographic determination of diclofenac in human plasma after solid-phase extraction

C. Arcelloni, R. Lanzi, S. Pedercini, G. Molteni, I. Fermo, A. Pontiroli, R. Paroni

Research output: Contribution to journalArticlepeer-review

Abstract

A novel high-performance liquid chromatographic (HPLC) method for the quantification of diclofenac in human plasma was set up. Samples, added with ibuprofen (used as internal standard) were purified by solid-phase extraction using Abselut Nexus cartridges (Varian) not requiring pre-conditioning. Drugs of interest were eluted directly into the autosampler vials and injected. The recovery of diclofenac was 92%, the analysis lasted 7 min with a sensitivity of 5 ng/ml and intra- and inter-day RSDs of 3 and 8%, respectively. The pharmacokinetics of diclofenac after oral and rectal administration in 10 healthy volunteers are reported.

Original languageEnglish
Pages (from-to)195-200
Number of pages6
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume763
Issue number1-2
DOIs
Publication statusPublished - Nov 5 2001

Keywords

  • Diclofenac

ASJC Scopus subject areas

  • Chemistry(all)

Fingerprint Dive into the research topics of 'High-performance liquid chromatographic determination of diclofenac in human plasma after solid-phase extraction'. Together they form a unique fingerprint.

Cite this