High-performance liquid chromatographic determination of diclofenac in human plasma after solid-phase extraction

C. Arcelloni; R. Lanzi; S. Pedercini; G. Molteni; I. Fermo; A. Pontiroli; R. Paroni

doi:10.1016/S0378-4347(01)00383-8

High-performance liquid chromatographic determination of diclofenac in human plasma after solid-phase extraction

C. Arcelloni, R. Lanzi, S. Pedercini, G. Molteni, I. Fermo, A. Pontiroli, R. Paroni

Research output: Contribution to journal › Article › peer-review

Abstract

A novel high-performance liquid chromatographic (HPLC) method for the quantification of diclofenac in human plasma was set up. Samples, added with ibuprofen (used as internal standard) were purified by solid-phase extraction using Abselut Nexus cartridges (Varian) not requiring pre-conditioning. Drugs of interest were eluted directly into the autosampler vials and injected. The recovery of diclofenac was 92%, the analysis lasted 7 min with a sensitivity of 5 ng/ml and intra- and inter-day RSDs of 3 and 8%, respectively. The pharmacokinetics of diclofenac after oral and rectal administration in 10 healthy volunteers are reported.

Original language	English
Pages (from-to)	195-200
Number of pages	6
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	763
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-4347(01)00383-8
Publication status	Published - Nov 5 2001

Keywords

Diclofenac

ASJC Scopus subject areas

Chemistry(all)

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High-performance liquid chromatographic determination of diclofenac in human plasma after solid-phase extraction. / Arcelloni, C.; Lanzi, R.; Pedercini, S.; Molteni, G.; Fermo, I.; Pontiroli, A.; Paroni, R.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 763, No. 1-2, 05.11.2001, p. 195-200.

Research output: Contribution to journal › Article › peer-review

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AU - Pontiroli, A.

AU - Paroni, R.

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