High-performance liquid chromatographic determination of iothalamic acid in human plasma and urine

Flavio Gaspari, Laura Mainardi, Piero Ruggenenti, Giuseppe Remuzzi

Research output: Contribution to journalArticle


A simple, rapid and sensitive method for the determination of iothalamic acid (IA) in both plasma and urine is reported. After extraction with ethyl acetate, IA was determined by strong anion-exchange high-performance liquid chromatography with ultraviolet detection at 254 nm. The lower limit of detection was 0.5 μg/ml. The average recovery was 73 and 57% from plasma and urine, respectively. Linearity was found over the investigated concentration range (up to 500 μg/ml for plasma and up to 10.0 mg/ml for urine). The reproducibility of the technique was good (coefficient of variation less than 6%) as was the precision and accuracy (coefficient of variation less than 2.5%). No interference from endogenous substances or any of the common drugs tested was found.

Original languageEnglish
Pages (from-to)435-440
Number of pages6
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Issue number2
Publication statusPublished - Oct 4 1991


ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

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