High-performance liquid chromatographic purification and capillary electrophoresis quantification of the chemokine stromal cell-derived factor-1

Cinzia Arcelloni, Alessandro Aiuti, Arcadi Cipponi, Rita Paroni

Research output: Contribution to journalArticle

Abstract

Chemokines are members of the chemotactic cytokines family implicated in various immunoregulatory functions. The CXC-chemokine stromal cell derived factor-1 (SDF-1α) was purified from the culture medium of murine bone marrow stromal cell line (MS-5) by affinity and reversed-phase liquid chromatography. Yield and purity were assessed by capillary electrophoresis (CE) with reference to the human SDF-1α from recombinant DNA technology. CE technique was useful to evaluate the purity of human SDF-1α from chemical synthesis and to resolve murine and human SDF-1α, differing by only one amino acid. Chemotactic activity of the murine SDF-1α was tested on the basis of CE quantification. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)369-374
Number of pages6
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume729
Issue number1-2
DOIs
Publication statusPublished - Jun 11 1999

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Keywords

  • Chemokines
  • SDF-1α

ASJC Scopus subject areas

  • Chemistry(all)

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