Chemokines are members of the chemotactic cytokines family implicated in various immunoregulatory functions. The CXC-chemokine stromal cell derived factor-1 (SDF-1α) was purified from the culture medium of murine bone marrow stromal cell line (MS-5) by affinity and reversed-phase liquid chromatography. Yield and purity were assessed by capillary electrophoresis (CE) with reference to the human SDF-1α from recombinant DNA technology. CE technique was useful to evaluate the purity of human SDF-1α from chemical synthesis and to resolve murine and human SDF-1α, differing by only one amino acid. Chemotactic activity of the murine SDF-1α was tested on the basis of CE quantification. Copyright (C) 1999 Elsevier Science B.V.
|Number of pages||6|
|Journal||Journal of Chromatography B: Biomedical Sciences and Applications|
|Publication status||Published - Jun 11 1999|
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