High-performance liquid chromatography assay of N,N-dimethyl-p-toluidine released from bone cements: Evidence for toxicity

S. Stea; D. Granchi; C. Zolezzi; G. Ciapetti; M. Visentin; D. Cavedagna; A. Pizzoferrato

doi:10.1016/S0142-9612(96)00121-4

High-performance liquid chromatography assay of N,N-dimethyl-p-toluidine released from bone cements: Evidence for toxicity

S. Stea, D. Granchi, C. Zolezzi, G. Ciapetti, M. Visentin, D. Cavedagna, A. Pizzoferrato

Istituti Ortopedici Rizzoli

Research output: Contribution to journal › Article

Abstract

Five commercially available bone cements were analysed by high-performance liquid chromatography for detecting the residual content of an accelerator, the amine N,N-dimethyl-p-toluidine (DMPT), after curing. It was found that the concentration of DMPT in aqueous extracts decreases with time, being almost absent 7 days after curing. Differences were noticed among the cements; residual DMPT is higher in cements prepared with higher content of the amine. It is verified that DMPT's toxic effect on cell cultures is dose-related; a delay in the cell replication cycle is induced in vitro. Damage is reversible, thus justifying the low bone cement toxicity that is clinically ascertained.

Original language	English
Pages (from-to)	243-246
Number of pages	4
Journal	Biomaterials
Volume	18
Issue number	3
DOIs	https://doi.org/10.1016/S0142-9612(96)00121-4
Publication status	Published - Feb 1997

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Keywords

Biocompatibility
Cement
High-performance liquid chromatography
N,N-dimethyl-p-toluidine

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering

Cite this

Stea, S., Granchi, D., Zolezzi, C., Ciapetti, G., Visentin, M., Cavedagna, D., & Pizzoferrato, A. (1997). High-performance liquid chromatography assay of N,N-dimethyl-p-toluidine released from bone cements: Evidence for toxicity. Biomaterials, 18(3), 243-246. https://doi.org/10.1016/S0142-9612(96)00121-4

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AU - Cavedagna, D.

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10.1016/S0142-9612(96)00121-4