High-performance liquid chromatography assay of N,N-dimethyl-p-toluidine released from bone cements: Evidence for toxicity

S. Stea, D. Granchi, C. Zolezzi, G. Ciapetti, M. Visentin, D. Cavedagna, A. Pizzoferrato

Research output: Contribution to journalArticle


Five commercially available bone cements were analysed by high-performance liquid chromatography for detecting the residual content of an accelerator, the amine N,N-dimethyl-p-toluidine (DMPT), after curing. It was found that the concentration of DMPT in aqueous extracts decreases with time, being almost absent 7 days after curing. Differences were noticed among the cements; residual DMPT is higher in cements prepared with higher content of the amine. It is verified that DMPT's toxic effect on cell cultures is dose-related; a delay in the cell replication cycle is induced in vitro. Damage is reversible, thus justifying the low bone cement toxicity that is clinically ascertained.

Original languageEnglish
Pages (from-to)243-246
Number of pages4
Issue number3
Publication statusPublished - Feb 1997



  • Biocompatibility
  • Cement
  • High-performance liquid chromatography
  • N,N-dimethyl-p-toluidine

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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