TY - JOUR
T1 - High-performance liquid chromatography assay of N,N-dimethyl-p-toluidine released from bone cements
T2 - Evidence for toxicity
AU - Stea, S.
AU - Granchi, D.
AU - Zolezzi, C.
AU - Ciapetti, G.
AU - Visentin, M.
AU - Cavedagna, D.
AU - Pizzoferrato, A.
PY - 1997/2
Y1 - 1997/2
N2 - Five commercially available bone cements were analysed by high-performance liquid chromatography for detecting the residual content of an accelerator, the amine N,N-dimethyl-p-toluidine (DMPT), after curing. It was found that the concentration of DMPT in aqueous extracts decreases with time, being almost absent 7 days after curing. Differences were noticed among the cements; residual DMPT is higher in cements prepared with higher content of the amine. It is verified that DMPT's toxic effect on cell cultures is dose-related; a delay in the cell replication cycle is induced in vitro. Damage is reversible, thus justifying the low bone cement toxicity that is clinically ascertained.
AB - Five commercially available bone cements were analysed by high-performance liquid chromatography for detecting the residual content of an accelerator, the amine N,N-dimethyl-p-toluidine (DMPT), after curing. It was found that the concentration of DMPT in aqueous extracts decreases with time, being almost absent 7 days after curing. Differences were noticed among the cements; residual DMPT is higher in cements prepared with higher content of the amine. It is verified that DMPT's toxic effect on cell cultures is dose-related; a delay in the cell replication cycle is induced in vitro. Damage is reversible, thus justifying the low bone cement toxicity that is clinically ascertained.
KW - Biocompatibility
KW - Cement
KW - High-performance liquid chromatography
KW - N,N-dimethyl-p-toluidine
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U2 - 10.1016/S0142-9612(96)00121-4
DO - 10.1016/S0142-9612(96)00121-4
M3 - Article
C2 - 9031725
AN - SCOPUS:0031081433
VL - 18
SP - 243
EP - 246
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 3
ER -