High-performance liquid chromatography determination of pravastatin in plasma

I. Iacona, M. B. Regazzi, I. Buggia, P. Villani, V. Fiorito, M. Molinaro, E. Guarnone

Research output: Contribution to journalArticlepeer-review

Abstract

Pravastatin is a 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitor that reduces plasma cholesterol levels. Some analytical methods have been described for determination of pravastatin levels in biological fluids, but these methods are rather cumbersome and involve expensive specialized equipment, usually not available in a clinical setting. A new technique, re-versed-phase high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection capability, has been developed for the analytical determination of pravastatin in plasma. Extraction and sample clean-up procedures are simple and rapid to execute, yet yield chromatograms virtually free of interference from endogenous plasma constituents and other antihypercholester-olemic agents or drugs usually taken concomitantly with pravastatin. Our detection limit for pravastatin was 2 ng/ml. Standard curves were linear between 5 and 200 ng/ml, with a coefficient of variation (CV) of

Original languageEnglish
Pages (from-to)191-195
Number of pages5
JournalTherapeutic Drug Monitoring
Volume16
Issue number2
Publication statusPublished - 1994

Keywords

  • Cyclosporin A
  • Heart transplantation
  • High-performance liquid chromatography
  • Hypercholesterolemia
  • Pravastatin

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health
  • Toxicology

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