High-performance liquid chromatography-electrochemical detection of 3-methylhistidine in human urine

P. Betto, G. Ricciarello, S. Pichini, L. Dello Strologo, G. Rizzoni

Research output: Contribution to journalArticle

Abstract

A reversed-phase high-performance liquid chromatographic method is described for the determination of 3-methylhistidine content in human urine using pre-column derivatization with phenylisothiocyanate, isocratic elution with 15 mM sodium acetate-acetonitrile (92:8, v/v) and electrochemical detection. The limit of quantitation was 0.1 pmol. The method has been applied in routine analyses of 3-methylhistidine in both clinical and research work.

Original languageEnglish
Pages (from-to)256-260
Number of pages5
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume584
Issue number2
DOIs
Publication statusPublished - Dec 23 1992

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

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