High-performance liquid chromatography tandem mass spectrometry procedure with automated solid phase extraction sample preparation for the quantitative determination of paclitaxel (Taxol®) in human plasma

Cristina Sottani, Claudio Minoia, Maurizio D'Incalci, Massimiliano Paganini, Massimo Zucchetti

Research output: Contribution to journalArticlepeer-review

Abstract

A sensitive, specific, accurate and reproducible analytical method was developed and validated for the quantitation of the anticancer agent paclitaxel in human plasma. This procedure is based on high performance liquid chromatography/ion spray - tandem mass spectrometry. This methodology is highly specific because a MS/MS technique (multiple reactant-ion monitoring, MRM) was used for both paclitaxel and its internal standard. The use of a fully automated solid phase extraction procedure, using a CN Sep-pak cartridge, to improve the detection limit and quantification limit of paclitaxel in human plasma samples, was evaluated. The method involves the addition of methyl-paclitaxel as internal standard (I.S.). The retention times of paclitaxel and the I.S. were 2.8 and 4.0 min., respectively. The assay was linear over the range 5 to 500 ng/mL, with a quantification limit of 5 ng/mL having a coefficient of variation (C.V.)

Original languageEnglish
Pages (from-to)251-255
Number of pages5
JournalRapid Communications in Mass Spectrometry
Volume12
Issue number5
DOIs
Publication statusPublished - 1998

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Fingerprint

Dive into the research topics of 'High-performance liquid chromatography tandem mass spectrometry procedure with automated solid phase extraction sample preparation for the quantitative determination of paclitaxel (Taxol®) in human plasma'. Together they form a unique fingerprint.

Cite this