High Prevalence of Shared International Type 53 among Mycobacterium tuberculosis Complex Strains in Retreated Patients from Côte d'Ivoire

Timothée Ouassa, Emanuele Borroni, Guillaume Yao Loukou, Hortense Faye-Kette, Jacquemin Kouakou, Hervé Menan, Daniela Maria Cirillo

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Genotyping methods are useful tools to provide information on tuberculosis epidemic. They can allow a better response from health authorities and the implementation of measures for tuberculosis control. This study aimed to identify the main lineages and clades of Mycobacterium tuberculosis complex strains circulating in Côte d'Ivoire. Methods/Main Findings: Strains isolated from sputum samples of patients ongoing retreatment from all the country were characterized by spoligotyping and by MIRU-VNTR. Profiles obtained by spoligotyping were first compared to the SITVIT/SpolDB4 database for family assignment. Of 194 strains analysed, 146 (75.3%) belonged to the T lineage. The most predominant spoligotype was the shared international type 53 with 135 strains (69.6%). In contrast with neighbouring countries, LAM (11 strains, 5.7%) and H (9 strains 4.6%) lineages were slightly represented. Only 3 Beijing strains (1.5%) and 4 strains of Mycobacterium africanum (2%) were found. Analysis of the results obtained with MIRU-VNTR revealed also a high level of clustering. Conclusion/Significance: The population of Mycobacterium tuberculosis complex strains among retreatment cases in Côte d'Ivoire exhibits a low diversity, allowing to assume recent transmission and locally based infection.

Original languageEnglish
Article numbere45363
JournalPLoS One
Volume7
Issue number9
DOIs
Publication statusPublished - Sep 18 2012

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Mycobacterium tuberculosis complex
Retreatment
Mycobacterium tuberculosis
Tuberculosis
Mycobacterium
Sputum
Cluster Analysis
Databases
Health
minisatellite repeats
Infection
Population
tuberculosis
Mycobacterium africanum
genotyping
Beijing
lipoarabinomannan

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

High Prevalence of Shared International Type 53 among Mycobacterium tuberculosis Complex Strains in Retreated Patients from Côte d'Ivoire. / Ouassa, Timothée; Borroni, Emanuele; Loukou, Guillaume Yao; Faye-Kette, Hortense; Kouakou, Jacquemin; Menan, Hervé; Cirillo, Daniela Maria.

In: PLoS One, Vol. 7, No. 9, e45363, 18.09.2012.

Research output: Contribution to journalArticle

Ouassa, Timothée ; Borroni, Emanuele ; Loukou, Guillaume Yao ; Faye-Kette, Hortense ; Kouakou, Jacquemin ; Menan, Hervé ; Cirillo, Daniela Maria. / High Prevalence of Shared International Type 53 among Mycobacterium tuberculosis Complex Strains in Retreated Patients from Côte d'Ivoire. In: PLoS One. 2012 ; Vol. 7, No. 9.
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