High-resolution genome-wide array comparative genomic hybridization in splenic marginal zone B-cell lymphoma

Francesca Novara, Luca Arcaini, Michele Merli, Francesco Passamonti, Silvia Zibellini, Silvia Rizzi, Sara Rattotti, Elisa Rumi, Cristiana Pascutto, Annalisa Vetro, Cesare Astori, Emanuela Boveri, Marco Lucioni, Marco Paulli, Orsetta Zuffardi, Mario Lazzarino

Research output: Contribution to journalArticle

Abstract

Splenic marginal zone B-cell lymphoma is characterized by high genetic heterogeneity, and hepatitis C virus infection seems to be involved in a subset of patients. The aims of the analysis were to identify potential genetic alterations related to hepatitis C virus status, IgVH gene mutational status, and prognostic categories identified in a multicenter study (Blood 2006;107:4643). Genome-wide array comparative genomic hybridization at a 100-kilobase (kb) resolution was performed in 34 patients with splenic marginal zone B-cell lymphoma, 12 of whom were hepatitis C virus positive. Array-comparative genomic hybridization experiments revealed no copy number alterations in 10 patients (4 were hepatitis C virus positive). A median of 5.6 and 3.8 copy number alterations were detected in hepatitis C virus-positive and in hepatitis C virus-negative patients, respectively. The most frequent copy number alterations involved chromosomes 7 and 17 (21% and 24%, respectively). Except for Xp gain (P = .01), no differences in common alterations were found between hepatitis C virus-positive and hepatitis C virus-negative cases. Unmutated status of the IgVH gene was related to del(7q) (P = .04) and dup(12q) (P = .03). The high-risk group identified according to the new splenic marginal zone B-cell lymphoma prognostic score was associated with del(7q) (P = .01) and del(17p) (P = .02). Hepatitis C virus-positive splenic marginal zone B-cell lymphoma patients have no specific chromosome alterations. Patients with poor prognosis are characterized by distinctive imbalances.

Original languageEnglish
Pages (from-to)1628-1637
Number of pages10
JournalHuman Pathology
Volume40
Issue number11
DOIs
Publication statusPublished - Nov 2009

Keywords

  • Array-CGH
  • Hepatitis C virus
  • Splenic marginal zone lymphoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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