High resolution in vitro bioluminescence imaging using a multimodal optical system

L. Altabella, C. R. Gigliotti, Laura Perani, M. P. Crippa, F. Boschi, A. E. Spinelli

Research output: Contribution to journalArticle


Bioluminescence in vitro studies are usually performed with dedicated microscopes. In this work, we developed a novel image recovery algorithm and a multimodal system prototype to perform bioluminescence microscopy. We performed a feasibility study using GEANT4 Monte Carlo (MC) simulation of bioluminescent cells acquired at low SNR frames and processed using a Super Resolution Regularization Algorithm (SRRA). The method was also tested using in vitro cell acquisition. The results obtained with MC simulations showed an improvement in the spatial resolution from 90 μ m to 10 μ m and from 110 μ m to 13 μ m for in vitro imaging of mesothelioma cells.

Original languageEnglish
Article numberC01035
JournalJournal of Instrumentation
Issue number1
Publication statusPublished - Jan 18 2016



  • Image processing
  • Multi-modality systems

ASJC Scopus subject areas

  • Instrumentation
  • Mathematical Physics

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