High resolution in vitro bioluminescence imaging using a multimodal optical system

L. Altabella, C. R. Gigliotti, Laura Perani, M. P. Crippa, F. Boschi, A. E. Spinelli

Research output: Contribution to journalArticle

Abstract

Bioluminescence in vitro studies are usually performed with dedicated microscopes. In this work, we developed a novel image recovery algorithm and a multimodal system prototype to perform bioluminescence microscopy. We performed a feasibility study using GEANT4 Monte Carlo (MC) simulation of bioluminescent cells acquired at low SNR frames and processed using a Super Resolution Regularization Algorithm (SRRA). The method was also tested using in vitro cell acquisition. The results obtained with MC simulations showed an improvement in the spatial resolution from 90 μ m to 10 μ m and from 110 μ m to 13 μ m for in vitro imaging of mesothelioma cells.

Original languageEnglish
Article numberC01035
JournalJournal of Instrumentation
Volume11
Issue number1
DOIs
Publication statusPublished - Jan 18 2016

Fingerprint

Bioluminescence
bioluminescence
Optical systems
Optical System
High Resolution
Imaging
Imaging techniques
high resolution
Cell
Monte Carlo Simulation
cells
Image Recovery
Microscopic examination
Microscopes
Super-resolution
Recovery
Microscopy
Spatial Resolution
Microscope
Regularization

Keywords

  • Image processing
  • Multi-modality systems

ASJC Scopus subject areas

  • Instrumentation
  • Mathematical Physics

Cite this

High resolution in vitro bioluminescence imaging using a multimodal optical system. / Altabella, L.; Gigliotti, C. R.; Perani, Laura; Crippa, M. P.; Boschi, F.; Spinelli, A. E.

In: Journal of Instrumentation, Vol. 11, No. 1, C01035, 18.01.2016.

Research output: Contribution to journalArticle

Altabella, L. ; Gigliotti, C. R. ; Perani, Laura ; Crippa, M. P. ; Boschi, F. ; Spinelli, A. E. / High resolution in vitro bioluminescence imaging using a multimodal optical system. In: Journal of Instrumentation. 2016 ; Vol. 11, No. 1.
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