High sensitivity and specificity assay for detection of leukemia/lymphoma cells in human bone marrow

M. Bregni, S. Siena, R. Dalla-Favera, A. M. Gianni

Research output: Contribution to journalArticle

Abstract

An assay of high sensitivity and specificity for detection of residual malignant cells in remission bone marrow from patients with acute lymphoblastic leukemia (ALL) and non-Hodgkin's lymphoma (NHL) has been developed. The assay combined an immunoselection step with immunoglobulin gene rearrangement analysis by high specific-activity DNA Probes. In experimental conditions, less than one contaminating tumor cell out of 1000 bone marrow cells was detected. A bone marrow contamination was detected in morphologically negative bone marrow specimens from two intermediate-grate non-Hodgkin's lymphoma patients. This method can be of value in determining a true remission status in high-risk ALL and NHL patients.

Original languageEnglish
Pages (from-to)473-482
Number of pages10
JournalAnnals of the New York Academy of Sciences
Volume511
Publication statusPublished - 1987

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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