High sensitivity optical microscppe for single molecule spectroscopy studies

Gabriele Malengo; Roberto Milani; Fabio Cannone; Silke Krol; Alberto Diaspro; Giuseppe Chirico

doi:10.1063/1.1777385

High sensitivity optical microscppe for single molecule spectroscopy studies

Gabriele Malengo, Roberto Milani, Fabio Cannone, Silke Krol, Alberto Diaspro, Giuseppe Chirico

IRCCS Giovanni Paolo II

Research output: Contribution to journal › Article › peer-review

Abstract

The single molecule emission with 50 ms time resolution was investigated with a closed loop piezo actuator and detection was performed through avalanche photodiodes. The two algorithms was used for fast imaging in raster scanning two photon microscope. A fluorescence fluctution analysis was implemented in slow scanning algorithm by computing the photon counting histogram (PCH) on each pixel of the image. The point spread function of the setup was measured and applications to cell imaging and single dyes were described.

Original language	English
Pages (from-to)	2746-2751
Number of pages	6
Journal	Review of Scientific Instruments
Volume	75
Issue number	8
DOIs	https://doi.org/10.1063/1.1777385
Publication status	Published - Aug 2004

ASJC Scopus subject areas

Instrumentation
Physics and Astronomy (miscellaneous)

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10.1063/1.1777385

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title = "High sensitivity optical microscppe for single molecule spectroscopy studies",

abstract = "The single molecule emission with 50 ms time resolution was investigated with a closed loop piezo actuator and detection was performed through avalanche photodiodes. The two algorithms was used for fast imaging in raster scanning two photon microscope. A fluorescence fluctution analysis was implemented in slow scanning algorithm by computing the photon counting histogram (PCH) on each pixel of the image. The point spread function of the setup was measured and applications to cell imaging and single dyes were described.",

author = "Gabriele Malengo and Roberto Milani and Fabio Cannone and Silke Krol and Alberto Diaspro and Giuseppe Chirico",

year = "2004",

month = aug,

doi = "10.1063/1.1777385",

language = "English",

volume = "75",

pages = "2746--2751",

journal = "Review of Scientific Instruments",

issn = "0034-6748",

publisher = "American Institute of Physics Publising LLC",

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T1 - High sensitivity optical microscppe for single molecule spectroscopy studies

AU - Malengo, Gabriele

AU - Milani, Roberto

AU - Cannone, Fabio

AU - Krol, Silke

AU - Diaspro, Alberto

AU - Chirico, Giuseppe

PY - 2004/8

Y1 - 2004/8

N2 - The single molecule emission with 50 ms time resolution was investigated with a closed loop piezo actuator and detection was performed through avalanche photodiodes. The two algorithms was used for fast imaging in raster scanning two photon microscope. A fluorescence fluctution analysis was implemented in slow scanning algorithm by computing the photon counting histogram (PCH) on each pixel of the image. The point spread function of the setup was measured and applications to cell imaging and single dyes were described.

AB - The single molecule emission with 50 ms time resolution was investigated with a closed loop piezo actuator and detection was performed through avalanche photodiodes. The two algorithms was used for fast imaging in raster scanning two photon microscope. A fluorescence fluctution analysis was implemented in slow scanning algorithm by computing the photon counting histogram (PCH) on each pixel of the image. The point spread function of the setup was measured and applications to cell imaging and single dyes were described.

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DO - 10.1063/1.1777385

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High sensitivity optical microscppe for single molecule spectroscopy studies

Abstract

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