Abstract
Normal brain tissue from 28 individuals and 50 glioma samples were analyzed by real-time Quantitative Methylation-Specific PCR (QMSP). Data from this analysis were compared with results obtained on the same samples by MSP. QMSP analysis demonstrated a statistically significant difference in both methylation level (P =.000009 Mann Whitney Test) and frequencies (P =.0000007, Z-test) in tumour samples as compared with normal brain tissues. Although QMSP and MSP showed similar sensitivity, the specificity of QMSP analysis was significantly higher (93; CI95: 84-100) as compared with MSP (64; 95CI: 46-82). Our results suggest that QMSP analysis may represent a powerful tool to identify glioma patients that will benefit from alkylating agents chemotherapy.
Original language | English |
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Article number | 531692 |
Journal | Journal of Biomedicine and Biotechnology |
Volume | 2009 |
DOIs | |
Publication status | Published - 2009 |
ASJC Scopus subject areas
- Biotechnology
- Molecular Medicine
- Genetics
- Molecular Biology
- Health, Toxicology and Mutagenesis
- Medicine(all)