High specificity of quantitative methylation-specific pcr analysis for mgmt promoter hypermethylation detection in gliomas

Paola Parrella; Antonella La Torre; Massimiliano Copetti; Vanna M. Valori; Raffaela Barbano; Angelo Notarangelo; Michele Bisceglia; Antonietta Pia Gallo; Teresa Balsamo; Maria Luana Poeta; Massimo Carella; Domenico Catapano; Salvatore Parisi; Bruno Dallapiccola; Evaristo Maiello; Vincenzo D'Angelo; Vito Michele Fazio

doi:10.1155/2009/531692

High specificity of quantitative methylation-specific pcr analysis for mgmt promoter hypermethylation detection in gliomas

Paola Parrella, Antonella La Torre, Massimiliano Copetti, Vanna M. Valori, Raffaela Barbano, Angelo Notarangelo, Michele Bisceglia, Antonietta Pia Gallo, Teresa Balsamo, Maria Luana Poeta, Massimo Carella, Domenico Catapano, Salvatore Parisi, Bruno Dallapiccola, Evaristo Maiello, Vincenzo D'Angelo, Vito Michele Fazio

Research output: Contribution to journal › Article › peer-review

Abstract

Normal brain tissue from 28 individuals and 50 glioma samples were analyzed by real-time Quantitative Methylation-Specific PCR (QMSP). Data from this analysis were compared with results obtained on the same samples by MSP. QMSP analysis demonstrated a statistically significant difference in both methylation level (P =.000009 Mann Whitney Test) and frequencies (P =.0000007, Z-test) in tumour samples as compared with normal brain tissues. Although QMSP and MSP showed similar sensitivity, the specificity of QMSP analysis was significantly higher (93; CI95: 84-100) as compared with MSP (64; 95CI: 46-82). Our results suggest that QMSP analysis may represent a powerful tool to identify glioma patients that will benefit from alkylating agents chemotherapy.

Original language	English
Article number	531692
Journal	Journal of Biomedicine and Biotechnology
Volume	2009
DOIs	https://doi.org/10.1155/2009/531692
Publication status	Published - 2009

ASJC Scopus subject areas

Biotechnology
Molecular Medicine
Genetics
Molecular Biology
Health, Toxicology and Mutagenesis
Medicine(all)

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10.1155/2009/531692

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Parrella, P., La Torre, A., Copetti, M., Valori, V. M., Barbano, R., Notarangelo, A., Bisceglia, M., Gallo, A. P., Balsamo, T., Poeta, M. L., Carella, M., Catapano, D., Parisi, S., Dallapiccola, B., Maiello, E., D'Angelo, V., & Fazio, V. M. (2009). High specificity of quantitative methylation-specific pcr analysis for mgmt promoter hypermethylation detection in gliomas. Journal of Biomedicine and Biotechnology, 2009, [531692]. https://doi.org/10.1155/2009/531692

High specificity of quantitative methylation-specific pcr analysis for mgmt promoter hypermethylation detection in gliomas. / Parrella, Paola; La Torre, Antonella; Copetti, Massimiliano; Valori, Vanna M.; Barbano, Raffaela; Notarangelo, Angelo; Bisceglia, Michele; Gallo, Antonietta Pia; Balsamo, Teresa; Poeta, Maria Luana; Carella, Massimo; Catapano, Domenico; Parisi, Salvatore; Dallapiccola, Bruno ; Maiello, Evaristo; D'Angelo, Vincenzo; Fazio, Vito Michele.

In: Journal of Biomedicine and Biotechnology, Vol. 2009, 531692, 2009.

Research output: Contribution to journal › Article › peer-review

Parrella, P, La Torre, A, Copetti, M, Valori, VM, Barbano, R, Notarangelo, A, Bisceglia, M, Gallo, AP, Balsamo, T, Poeta, ML, Carella, M, Catapano, D, Parisi, S, Dallapiccola, B , Maiello, E, D'Angelo, V & Fazio, VM 2009, 'High specificity of quantitative methylation-specific pcr analysis for mgmt promoter hypermethylation detection in gliomas', Journal of Biomedicine and Biotechnology, vol. 2009, 531692. https://doi.org/10.1155/2009/531692

Parrella, Paola ; La Torre, Antonella ; Copetti, Massimiliano ; Valori, Vanna M. ; Barbano, Raffaela ; Notarangelo, Angelo ; Bisceglia, Michele ; Gallo, Antonietta Pia ; Balsamo, Teresa ; Poeta, Maria Luana ; Carella, Massimo ; Catapano, Domenico ; Parisi, Salvatore ; Dallapiccola, Bruno ; Maiello, Evaristo ; D'Angelo, Vincenzo ; Fazio, Vito Michele. / High specificity of quantitative methylation-specific pcr analysis for mgmt promoter hypermethylation detection in gliomas. In: Journal of Biomedicine and Biotechnology. 2009 ; Vol. 2009.

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title = "High specificity of quantitative methylation-specific pcr analysis for mgmt promoter hypermethylation detection in gliomas",

abstract = "Normal brain tissue from 28 individuals and 50 glioma samples were analyzed by real-time Quantitative Methylation-Specific PCR (QMSP). Data from this analysis were compared with results obtained on the same samples by MSP. QMSP analysis demonstrated a statistically significant difference in both methylation level (P =.000009 Mann Whitney Test) and frequencies (P =.0000007, Z-test) in tumour samples as compared with normal brain tissues. Although QMSP and MSP showed similar sensitivity, the specificity of QMSP analysis was significantly higher (93; CI95: 84-100) as compared with MSP (64; 95CI: 46-82). Our results suggest that QMSP analysis may represent a powerful tool to identify glioma patients that will benefit from alkylating agents chemotherapy.",

author = "Paola Parrella and {La Torre}, Antonella and Massimiliano Copetti and Valori, {Vanna M.} and Raffaela Barbano and Angelo Notarangelo and Michele Bisceglia and Gallo, {Antonietta Pia} and Teresa Balsamo and Poeta, {Maria Luana} and Massimo Carella and Domenico Catapano and Salvatore Parisi and Bruno Dallapiccola and Evaristo Maiello and Vincenzo D'Angelo and Fazio, {Vito Michele}",

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AU - Barbano, Raffaela

AU - Notarangelo, Angelo

AU - Bisceglia, Michele

AU - Gallo, Antonietta Pia

AU - Balsamo, Teresa

AU - Poeta, Maria Luana

AU - Carella, Massimo

AU - Catapano, Domenico

AU - Parisi, Salvatore

AU - Dallapiccola, Bruno

AU - Maiello, Evaristo

AU - D'Angelo, Vincenzo

AU - Fazio, Vito Michele

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AB - Normal brain tissue from 28 individuals and 50 glioma samples were analyzed by real-time Quantitative Methylation-Specific PCR (QMSP). Data from this analysis were compared with results obtained on the same samples by MSP. QMSP analysis demonstrated a statistically significant difference in both methylation level (P =.000009 Mann Whitney Test) and frequencies (P =.0000007, Z-test) in tumour samples as compared with normal brain tissues. Although QMSP and MSP showed similar sensitivity, the specificity of QMSP analysis was significantly higher (93; CI95: 84-100) as compared with MSP (64; 95CI: 46-82). Our results suggest that QMSP analysis may represent a powerful tool to identify glioma patients that will benefit from alkylating agents chemotherapy.

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High specificity of quantitative methylation-specific pcr analysis for mgmt promoter hypermethylation detection in gliomas

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