High specificity of quantitative methylation-specific pcr analysis for mgmt promoter hypermethylation detection in gliomas

Paola Parrella, Antonella La Torre, Massimiliano Copetti, Vanna M. Valori, Raffaela Barbano, Angelo Notarangelo, Michele Bisceglia, Antonietta Pia Gallo, Teresa Balsamo, Maria Luana Poeta, Massimo Carella, Domenico Catapano, Salvatore Parisi, Bruno Dallapiccola, Evaristo Maiello, Vincenzo D'Angelo, Vito Michele Fazio

Research output: Contribution to journalArticlepeer-review

Abstract

Normal brain tissue from 28 individuals and 50 glioma samples were analyzed by real-time Quantitative Methylation-Specific PCR (QMSP). Data from this analysis were compared with results obtained on the same samples by MSP. QMSP analysis demonstrated a statistically significant difference in both methylation level (P =.000009 Mann Whitney Test) and frequencies (P =.0000007, Z-test) in tumour samples as compared with normal brain tissues. Although QMSP and MSP showed similar sensitivity, the specificity of QMSP analysis was significantly higher (93; CI95: 84-100) as compared with MSP (64; 95CI: 46-82). Our results suggest that QMSP analysis may represent a powerful tool to identify glioma patients that will benefit from alkylating agents chemotherapy.

Original languageEnglish
Article number531692
JournalJournal of Biomedicine and Biotechnology
Volume2009
DOIs
Publication statusPublished - 2009

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Genetics
  • Molecular Biology
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

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