TY - GEN
T1 - High-Throughput analysis of the drug mode of action of PB28, MC18 and MC70, three cyclohexylpiperazine derivative new molecules
AU - Bevilacqua, Vitoantonio
AU - Pannarale, Paolo
AU - Mastronardi, Giuseppe
AU - Azzariti, Amalia
AU - Tommasi, Stefania
AU - Menolascina, Filippo
AU - Iorio, Francesco
AU - Di Bernardo, Diego
AU - Paradiso, Angelo
AU - Colabufo, Nicola A.
AU - Berardi, Francesco
AU - Perrone, Roberto
AU - Tagliaferri, Roberto
PY - 2008
Y1 - 2008
N2 - Objective: This work explores the mode of action of PB28, MC70 and MC18 three molecules that showed anti-tumoral properties by arresting cellular growth and inhibiting glycoprotein P. Methods: Here we conduct a microarray-based study and analyze the expression patterns associated with the action of drugs. An ontology based analysis has been conducted, and the individuated cellular processes have been analyzed with gene networks, examining the interactions among genes. A clustering analysis revealed mechanisms shared with other drugs. Results: The results indicate that this compounds have side effects that include inflammatory response and fever, induced by the interleukin signaling pathway. Other evidences related with known effects of the compounds were highlighted. Conclusions: The results indicate that the direct effects could be reached at a post-transcriptional level of P-gp or through other targets, further studies will address these hypothesis. The prediction of side effects will be useful in subsequent in vivo experiments.
AB - Objective: This work explores the mode of action of PB28, MC70 and MC18 three molecules that showed anti-tumoral properties by arresting cellular growth and inhibiting glycoprotein P. Methods: Here we conduct a microarray-based study and analyze the expression patterns associated with the action of drugs. An ontology based analysis has been conducted, and the individuated cellular processes have been analyzed with gene networks, examining the interactions among genes. A clustering analysis revealed mechanisms shared with other drugs. Results: The results indicate that this compounds have side effects that include inflammatory response and fever, induced by the interleukin signaling pathway. Other evidences related with known effects of the compounds were highlighted. Conclusions: The results indicate that the direct effects could be reached at a post-transcriptional level of P-gp or through other targets, further studies will address these hypothesis. The prediction of side effects will be useful in subsequent in vivo experiments.
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U2 - 10.1007/978-3-540-85984-0_130
DO - 10.1007/978-3-540-85984-0_130
M3 - Conference contribution
AN - SCOPUS:53049106081
SN - 3540859837
SN - 9783540859833
VL - 5227 LNAI
T3 - Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
SP - 1085
EP - 1092
BT - Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
T2 - 4th International Conference on Intelligent Computing, ICIC 2008
Y2 - 15 September 2008 through 18 September 2008
ER -