High-throughput microRNA profiling of pediatric high-grade gliomas

Evelina Miele, Francesca Romana Buttarelli, Antonella Arcella, Federica Begalli, Neha Garg, Marianna Silvano, Agnese Po, Caterina Baldi, Giuseppe Carissimo, Manila Antonelli, Gian Paolo Spinelli, Carlo Capalbo, Vittoria Donofrio, Isabella Morra, Paolo Nozza, Alberto Gulino, Felice Giangaspero, Elisabetta Ferretti

Research output: Contribution to journalArticle

Abstract

BackgroundHigh-grade gliomas (HGGs) account for 15% of all pediatric brain tumors and are a leading cause of cancer-related mortality and morbidity. Pediatric HGGs (pHGGs) are histologically indistinguishable from their counterpart in adulthood. However, recent investigations indicate that differences occur at the molecular level, thus suggesting that the molecular path to gliomagenesis in childhood is distinct from that of adults. MicroRNAs (miRNAs) have been identified as key molecules in gene expression regulation, both in development and in cancer. miRNAs have been investigated in adult high-grade gliomas (aHGGs), but scant information is available for pHGGs.MethodsWe explored the differences in microRNAs between pHGG and aHGG, in both fresh-frozen and paraffin-embedded tissue, by high-throughput miRNA profiling. We also evaluated the biological effects of miR-17-92 cluster silencing on a pHGG cell line.ResultsComparison of miRNA expression patterns in formalin versus frozen specimens resulted in high correlation between both types of samples. The analysis of miRNA profiling revealed a specific microRNA pattern in pHGG with an overexpression and a proliferative role of the miR-17-92 cluster. Moreover, we highlighted a possible quenching function of miR-17-92 cluster on its target gene PTEN, together with an activation of tumorigenic signaling such as sonic hedgehog in pHGG.ConclusionsOur results suggest that microRNA profiling represents a tool to distinguishing pediatric from adult HGG and that miR-17-92 cluster sustains pHGG.

Original languageEnglish
Pages (from-to)228-240
Number of pages13
JournalNeuro-Oncology
Volume16
Issue number2
DOIs
Publication statusPublished - Jan 2014

Fingerprint

MicroRNAs
Glioma
Pediatrics
Gene Expression Regulation
Brain Neoplasms
Paraffin
Formaldehyde
Neoplasms
Morbidity
Cell Line
Mortality
Genes

Keywords

  • cancer
  • expression profiling
  • microRNA
  • pediatric gliomas

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Clinical Neurology

Cite this

Miele, E., Buttarelli, F. R., Arcella, A., Begalli, F., Garg, N., Silvano, M., ... Ferretti, E. (2014). High-throughput microRNA profiling of pediatric high-grade gliomas. Neuro-Oncology, 16(2), 228-240. https://doi.org/10.1093/neuonc/not215

High-throughput microRNA profiling of pediatric high-grade gliomas. / Miele, Evelina; Buttarelli, Francesca Romana; Arcella, Antonella; Begalli, Federica; Garg, Neha; Silvano, Marianna; Po, Agnese; Baldi, Caterina; Carissimo, Giuseppe; Antonelli, Manila; Spinelli, Gian Paolo; Capalbo, Carlo; Donofrio, Vittoria; Morra, Isabella; Nozza, Paolo; Gulino, Alberto; Giangaspero, Felice; Ferretti, Elisabetta.

In: Neuro-Oncology, Vol. 16, No. 2, 01.2014, p. 228-240.

Research output: Contribution to journalArticle

Miele, E, Buttarelli, FR, Arcella, A, Begalli, F, Garg, N, Silvano, M, Po, A, Baldi, C, Carissimo, G, Antonelli, M, Spinelli, GP, Capalbo, C, Donofrio, V, Morra, I, Nozza, P, Gulino, A, Giangaspero, F & Ferretti, E 2014, 'High-throughput microRNA profiling of pediatric high-grade gliomas', Neuro-Oncology, vol. 16, no. 2, pp. 228-240. https://doi.org/10.1093/neuonc/not215
Miele E, Buttarelli FR, Arcella A, Begalli F, Garg N, Silvano M et al. High-throughput microRNA profiling of pediatric high-grade gliomas. Neuro-Oncology. 2014 Jan;16(2):228-240. https://doi.org/10.1093/neuonc/not215
Miele, Evelina ; Buttarelli, Francesca Romana ; Arcella, Antonella ; Begalli, Federica ; Garg, Neha ; Silvano, Marianna ; Po, Agnese ; Baldi, Caterina ; Carissimo, Giuseppe ; Antonelli, Manila ; Spinelli, Gian Paolo ; Capalbo, Carlo ; Donofrio, Vittoria ; Morra, Isabella ; Nozza, Paolo ; Gulino, Alberto ; Giangaspero, Felice ; Ferretti, Elisabetta. / High-throughput microRNA profiling of pediatric high-grade gliomas. In: Neuro-Oncology. 2014 ; Vol. 16, No. 2. pp. 228-240.
@article{4829b8f2ac6d4ad1adbbb6b42bd08e04,
title = "High-throughput microRNA profiling of pediatric high-grade gliomas",
abstract = "BackgroundHigh-grade gliomas (HGGs) account for 15{\%} of all pediatric brain tumors and are a leading cause of cancer-related mortality and morbidity. Pediatric HGGs (pHGGs) are histologically indistinguishable from their counterpart in adulthood. However, recent investigations indicate that differences occur at the molecular level, thus suggesting that the molecular path to gliomagenesis in childhood is distinct from that of adults. MicroRNAs (miRNAs) have been identified as key molecules in gene expression regulation, both in development and in cancer. miRNAs have been investigated in adult high-grade gliomas (aHGGs), but scant information is available for pHGGs.MethodsWe explored the differences in microRNAs between pHGG and aHGG, in both fresh-frozen and paraffin-embedded tissue, by high-throughput miRNA profiling. We also evaluated the biological effects of miR-17-92 cluster silencing on a pHGG cell line.ResultsComparison of miRNA expression patterns in formalin versus frozen specimens resulted in high correlation between both types of samples. The analysis of miRNA profiling revealed a specific microRNA pattern in pHGG with an overexpression and a proliferative role of the miR-17-92 cluster. Moreover, we highlighted a possible quenching function of miR-17-92 cluster on its target gene PTEN, together with an activation of tumorigenic signaling such as sonic hedgehog in pHGG.ConclusionsOur results suggest that microRNA profiling represents a tool to distinguishing pediatric from adult HGG and that miR-17-92 cluster sustains pHGG.",
keywords = "cancer, expression profiling, microRNA, pediatric gliomas",
author = "Evelina Miele and Buttarelli, {Francesca Romana} and Antonella Arcella and Federica Begalli and Neha Garg and Marianna Silvano and Agnese Po and Caterina Baldi and Giuseppe Carissimo and Manila Antonelli and Spinelli, {Gian Paolo} and Carlo Capalbo and Vittoria Donofrio and Isabella Morra and Paolo Nozza and Alberto Gulino and Felice Giangaspero and Elisabetta Ferretti",
year = "2014",
month = "1",
doi = "10.1093/neuonc/not215",
language = "English",
volume = "16",
pages = "228--240",
journal = "Neuro-Oncology",
issn = "1522-8517",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - High-throughput microRNA profiling of pediatric high-grade gliomas

AU - Miele, Evelina

AU - Buttarelli, Francesca Romana

AU - Arcella, Antonella

AU - Begalli, Federica

AU - Garg, Neha

AU - Silvano, Marianna

AU - Po, Agnese

AU - Baldi, Caterina

AU - Carissimo, Giuseppe

AU - Antonelli, Manila

AU - Spinelli, Gian Paolo

AU - Capalbo, Carlo

AU - Donofrio, Vittoria

AU - Morra, Isabella

AU - Nozza, Paolo

AU - Gulino, Alberto

AU - Giangaspero, Felice

AU - Ferretti, Elisabetta

PY - 2014/1

Y1 - 2014/1

N2 - BackgroundHigh-grade gliomas (HGGs) account for 15% of all pediatric brain tumors and are a leading cause of cancer-related mortality and morbidity. Pediatric HGGs (pHGGs) are histologically indistinguishable from their counterpart in adulthood. However, recent investigations indicate that differences occur at the molecular level, thus suggesting that the molecular path to gliomagenesis in childhood is distinct from that of adults. MicroRNAs (miRNAs) have been identified as key molecules in gene expression regulation, both in development and in cancer. miRNAs have been investigated in adult high-grade gliomas (aHGGs), but scant information is available for pHGGs.MethodsWe explored the differences in microRNAs between pHGG and aHGG, in both fresh-frozen and paraffin-embedded tissue, by high-throughput miRNA profiling. We also evaluated the biological effects of miR-17-92 cluster silencing on a pHGG cell line.ResultsComparison of miRNA expression patterns in formalin versus frozen specimens resulted in high correlation between both types of samples. The analysis of miRNA profiling revealed a specific microRNA pattern in pHGG with an overexpression and a proliferative role of the miR-17-92 cluster. Moreover, we highlighted a possible quenching function of miR-17-92 cluster on its target gene PTEN, together with an activation of tumorigenic signaling such as sonic hedgehog in pHGG.ConclusionsOur results suggest that microRNA profiling represents a tool to distinguishing pediatric from adult HGG and that miR-17-92 cluster sustains pHGG.

AB - BackgroundHigh-grade gliomas (HGGs) account for 15% of all pediatric brain tumors and are a leading cause of cancer-related mortality and morbidity. Pediatric HGGs (pHGGs) are histologically indistinguishable from their counterpart in adulthood. However, recent investigations indicate that differences occur at the molecular level, thus suggesting that the molecular path to gliomagenesis in childhood is distinct from that of adults. MicroRNAs (miRNAs) have been identified as key molecules in gene expression regulation, both in development and in cancer. miRNAs have been investigated in adult high-grade gliomas (aHGGs), but scant information is available for pHGGs.MethodsWe explored the differences in microRNAs between pHGG and aHGG, in both fresh-frozen and paraffin-embedded tissue, by high-throughput miRNA profiling. We also evaluated the biological effects of miR-17-92 cluster silencing on a pHGG cell line.ResultsComparison of miRNA expression patterns in formalin versus frozen specimens resulted in high correlation between both types of samples. The analysis of miRNA profiling revealed a specific microRNA pattern in pHGG with an overexpression and a proliferative role of the miR-17-92 cluster. Moreover, we highlighted a possible quenching function of miR-17-92 cluster on its target gene PTEN, together with an activation of tumorigenic signaling such as sonic hedgehog in pHGG.ConclusionsOur results suggest that microRNA profiling represents a tool to distinguishing pediatric from adult HGG and that miR-17-92 cluster sustains pHGG.

KW - cancer

KW - expression profiling

KW - microRNA

KW - pediatric gliomas

UR - http://www.scopus.com/inward/record.url?scp=84893122766&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893122766&partnerID=8YFLogxK

U2 - 10.1093/neuonc/not215

DO - 10.1093/neuonc/not215

M3 - Article

C2 - 24305714

AN - SCOPUS:84893122766

VL - 16

SP - 228

EP - 240

JO - Neuro-Oncology

JF - Neuro-Oncology

SN - 1522-8517

IS - 2

ER -