High-throughput plasma docetaxel quantification by liquid chromatography-tandem mass spectrometry

Giuseppe Corona, Caterina Elia, Bruno Casetta, Sergio Frustaci, Giuseppe Toffoli

Research output: Contribution to journalArticle

Abstract

Background: The most valuable treatment option for breast, prostate and lung carcinomas is at present represented by a low dose of docetaxel, administered on a weekly basis. A better understanding of docetaxel pharmacokinetic and pharmacodynamic profiles could lead to an improvement in this dose regimen efficacy. Methods: In this study a high-throughput method is described for the rapid quantification of docetaxel for large clinical pharmacology investigations. This analytical approach is based on an automatic on-line purification and enrichment technique followed by a measurement in tandem mass spectrometry through Multiple Reaction Monitoring. Results: The assay was validated over a 0.15-1500ng/mL range. Intra-day precision ranged from 1.9% to 6.4%, while the inter-day was between 7.6% and 11.2%. The mean deviation from the nominal value ranged from -0.5% to 5.6% for the intra-day, and from -0.4% to 3.1% for the inter-day assay. Clinical applicability was demonstrated by measuring plasma pharmacokinetics in patients receiving weekly 25-35mg/m2 of docetaxel. Conclusion: The proposed LC-MS/MS assay was found to have a better performance than previously reported methods in terms of sensitivity and sample preparation. It does not require any laborious pre-analytical manipulation and can be easily employed in large clinical pharmacology studies.

Original languageEnglish
Pages (from-to)358-364
Number of pages7
JournalClinica Chimica Acta
Volume412
Issue number3-4
DOIs
Publication statusPublished - Jan 30 2011

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Keywords

  • Docetaxel
  • LC-MS/MS
  • On-line extraction
  • Therapeutic drug monitoring

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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