Abstract
Small molecules acting as selective activators (potentiators), inhibitors, or "correctors" of the CFTR chloride channel represent candidate drugs for various pathological conditions including cystic fibrosis and secretory diarrhea. The identification of CFTR pharmacological modulators may be achieved by screening highly diverse synthetic or natural compound libraries using high-throughput methods. A convenient assay for CFTR function is based on the halide sensitivity of the yellow fluorescent protein (YFP). CFTR activity can be simply assessed by measuring the rate of YFP signal decrease caused by iodide influx. This assay can be automated to test thousands of compounds per day.
| Original language | English |
|---|---|
| Pages (from-to) | 13-21 |
| Number of pages | 9 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 741 |
| DOIs | |
| Publication status | Published - 2011 |
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ASJC Scopus subject areas
- Medicine(all)
Cite this
High-throughput screening of libraries of compounds to identify CFTR modulators. / Pedemonte, Nicoletta; Zegarra-Moran, Olga; Galietta, Luis J V.
In: Methods in molecular biology (Clifton, N.J.), Vol. 741, 2011, p. 13-21.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High-throughput screening of libraries of compounds to identify CFTR modulators.
AU - Pedemonte, Nicoletta
AU - Zegarra-Moran, Olga
AU - Galietta, Luis J V
PY - 2011
Y1 - 2011
N2 - Small molecules acting as selective activators (potentiators), inhibitors, or "correctors" of the CFTR chloride channel represent candidate drugs for various pathological conditions including cystic fibrosis and secretory diarrhea. The identification of CFTR pharmacological modulators may be achieved by screening highly diverse synthetic or natural compound libraries using high-throughput methods. A convenient assay for CFTR function is based on the halide sensitivity of the yellow fluorescent protein (YFP). CFTR activity can be simply assessed by measuring the rate of YFP signal decrease caused by iodide influx. This assay can be automated to test thousands of compounds per day.
AB - Small molecules acting as selective activators (potentiators), inhibitors, or "correctors" of the CFTR chloride channel represent candidate drugs for various pathological conditions including cystic fibrosis and secretory diarrhea. The identification of CFTR pharmacological modulators may be achieved by screening highly diverse synthetic or natural compound libraries using high-throughput methods. A convenient assay for CFTR function is based on the halide sensitivity of the yellow fluorescent protein (YFP). CFTR activity can be simply assessed by measuring the rate of YFP signal decrease caused by iodide influx. This assay can be automated to test thousands of compounds per day.
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UR - http://www.scopus.com/inward/citedby.url?scp=80052311732&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-117-8_2
DO - 10.1007/978-1-61779-117-8_2
M3 - Article
VL - 741
SP - 13
EP - 21
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -