Higher placental anti-inflammatory IL-10 cytokine expression in HIV-1 infected women receiving longer zidovudine prophylaxis associated with nevirapine

Sakorn Pornprasert, Jean Yves Mary, Albert Faye, Pannee Leechanachai, Aram Limtrakul, Sungwal Rugpao, Pannee Sirivatanapa, Vorapin Gomuthbutra, Wanmanee Matanasaravoot, Sophie Le Cœur, Marc Lallemant, Françoise Barré-Sinoussi, Elisabeth Menu, Nicole Ngo-Giang-Huong, Ahidjo Ayouba, Sanupong Chailert, Gérard Chaouat, Muriel Derrien, Guillermina Dolcini, Nicole EtekiAnfumbom Jude Kfutwah, Odette Kouo, Brigitte Lemen, Juan Maldonado-Estrada, Eric Nerrienet, Bernadette Njinku, Gabriella Scarlatti, Mathurin Tejiokem, Gilbert Téné

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Placental cytokine balance may be critical for the control of mother-to-child transmission (MTCT) of HIV. We assessed whether the type and duration of antiretrovirals used for prevention of HIV-1-MTCT modified the inflammatory cytokine profile. We investigated the levels of cytokine expression in the placentas of 61 HIV-1-infected women who received zidovudine (ZDV) plus single dose nevirapine (SD-NVP) or ZDV only for prevention of MTCT. Placentas of 38 HIV-1-uninfected women were included as controls. All placentas were obtained after vaginal delivery. Levels of mRNA and cytokine expression were quantified using real-time PCR and ELISA, respectively, in placental explants and 24-hour culture supernatants and analyzed in relation to the women's characteristics and the type and duration of antiretroviral prophylaxis. HIV-1-infected and uninfected women did not show any differences in the expression of placental cytokine secretion except for a trend toward lower TNF-10 mRNA levels in HIV-1-infected women. Within the HIV-1-infected group, women who were exposed to a long duration of ZDV (>72 days) or received SD-NVP less than 5h prior to delivery, more frequently expressed detectable levels of IL-10 in their placentas (32% versus 7% (p = 0.01) and 32% versus 5% (p = 0.02), respectively). No infant was found to be HIV-1-infected. Our results showed a normalization of the placental cytokine balance in HIV-1-infected women receiving antiretroviral prophylaxis. Furthermore, the type and duration of antiretroviral prophylaxis have an impact on the placental anti-inflammatory IL-10 expression level, which may contribute to controlling HIV replication at the placental level, thus reducing MTCT of HIV-1.

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalCurrent HIV Research
Volume7
Issue number2
DOIs
Publication statusPublished - 2009

Fingerprint

Nevirapine
Zidovudine
Interleukin-10
HIV-1
Anti-Inflammatory Agents
Cytokines
Placenta
Mothers
HIV
Messenger RNA
Real-Time Polymerase Chain Reaction
Enzyme-Linked Immunosorbent Assay

Keywords

  • Antiretroviral prophylaxis
  • HIV-1
  • IL-10 cytokine
  • Mother-to-child transmission
  • Placenta
  • Zidovudine and nevirapine

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Higher placental anti-inflammatory IL-10 cytokine expression in HIV-1 infected women receiving longer zidovudine prophylaxis associated with nevirapine. / Pornprasert, Sakorn; Mary, Jean Yves; Faye, Albert; Leechanachai, Pannee; Limtrakul, Aram; Rugpao, Sungwal; Sirivatanapa, Pannee; Gomuthbutra, Vorapin; Matanasaravoot, Wanmanee; Le Cœur, Sophie; Lallemant, Marc; Barré-Sinoussi, Françoise; Menu, Elisabeth; Ngo-Giang-Huong, Nicole; Ayouba, Ahidjo; Chailert, Sanupong; Chaouat, Gérard; Derrien, Muriel; Dolcini, Guillermina; Eteki, Nicole; Kfutwah, Anfumbom Jude; Kouo, Odette; Lemen, Brigitte; Maldonado-Estrada, Juan; Nerrienet, Eric; Njinku, Bernadette; Scarlatti, Gabriella; Tejiokem, Mathurin; Téné, Gilbert.

In: Current HIV Research, Vol. 7, No. 2, 2009, p. 211-217.

Research output: Contribution to journalArticle

Pornprasert, S, Mary, JY, Faye, A, Leechanachai, P, Limtrakul, A, Rugpao, S, Sirivatanapa, P, Gomuthbutra, V, Matanasaravoot, W, Le Cœur, S, Lallemant, M, Barré-Sinoussi, F, Menu, E, Ngo-Giang-Huong, N, Ayouba, A, Chailert, S, Chaouat, G, Derrien, M, Dolcini, G, Eteki, N, Kfutwah, AJ, Kouo, O, Lemen, B, Maldonado-Estrada, J, Nerrienet, E, Njinku, B, Scarlatti, G, Tejiokem, M & Téné, G 2009, 'Higher placental anti-inflammatory IL-10 cytokine expression in HIV-1 infected women receiving longer zidovudine prophylaxis associated with nevirapine', Current HIV Research, vol. 7, no. 2, pp. 211-217. https://doi.org/10.2174/157016209787581472
Pornprasert, Sakorn ; Mary, Jean Yves ; Faye, Albert ; Leechanachai, Pannee ; Limtrakul, Aram ; Rugpao, Sungwal ; Sirivatanapa, Pannee ; Gomuthbutra, Vorapin ; Matanasaravoot, Wanmanee ; Le Cœur, Sophie ; Lallemant, Marc ; Barré-Sinoussi, Françoise ; Menu, Elisabeth ; Ngo-Giang-Huong, Nicole ; Ayouba, Ahidjo ; Chailert, Sanupong ; Chaouat, Gérard ; Derrien, Muriel ; Dolcini, Guillermina ; Eteki, Nicole ; Kfutwah, Anfumbom Jude ; Kouo, Odette ; Lemen, Brigitte ; Maldonado-Estrada, Juan ; Nerrienet, Eric ; Njinku, Bernadette ; Scarlatti, Gabriella ; Tejiokem, Mathurin ; Téné, Gilbert. / Higher placental anti-inflammatory IL-10 cytokine expression in HIV-1 infected women receiving longer zidovudine prophylaxis associated with nevirapine. In: Current HIV Research. 2009 ; Vol. 7, No. 2. pp. 211-217.
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AU - Leechanachai, Pannee

AU - Limtrakul, Aram

AU - Rugpao, Sungwal

AU - Sirivatanapa, Pannee

AU - Gomuthbutra, Vorapin

AU - Matanasaravoot, Wanmanee

AU - Le Cœur, Sophie

AU - Lallemant, Marc

AU - Barré-Sinoussi, Françoise

AU - Menu, Elisabeth

AU - Ngo-Giang-Huong, Nicole

AU - Ayouba, Ahidjo

AU - Chailert, Sanupong

AU - Chaouat, Gérard

AU - Derrien, Muriel

AU - Dolcini, Guillermina

AU - Eteki, Nicole

AU - Kfutwah, Anfumbom Jude

AU - Kouo, Odette

AU - Lemen, Brigitte

AU - Maldonado-Estrada, Juan

AU - Nerrienet, Eric

AU - Njinku, Bernadette

AU - Scarlatti, Gabriella

AU - Tejiokem, Mathurin

AU - Téné, Gilbert

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