Abstract
Placental cytokine balance may be critical for the control of mother-to-child transmission (MTCT) of HIV. We assessed whether the type and duration of antiretrovirals used for prevention of HIV-1-MTCT modified the inflammatory cytokine profile. We investigated the levels of cytokine expression in the placentas of 61 HIV-1-infected women who received zidovudine (ZDV) plus single dose nevirapine (SD-NVP) or ZDV only for prevention of MTCT. Placentas of 38 HIV-1-uninfected women were included as controls. All placentas were obtained after vaginal delivery. Levels of mRNA and cytokine expression were quantified using real-time PCR and ELISA, respectively, in placental explants and 24-hour culture supernatants and analyzed in relation to the women's characteristics and the type and duration of antiretroviral prophylaxis. HIV-1-infected and uninfected women did not show any differences in the expression of placental cytokine secretion except for a trend toward lower TNF-10 mRNA levels in HIV-1-infected women. Within the HIV-1-infected group, women who were exposed to a long duration of ZDV (>72 days) or received SD-NVP less than 5h prior to delivery, more frequently expressed detectable levels of IL-10 in their placentas (32% versus 7% (p = 0.01) and 32% versus 5% (p = 0.02), respectively). No infant was found to be HIV-1-infected. Our results showed a normalization of the placental cytokine balance in HIV-1-infected women receiving antiretroviral prophylaxis. Furthermore, the type and duration of antiretroviral prophylaxis have an impact on the placental anti-inflammatory IL-10 expression level, which may contribute to controlling HIV replication at the placental level, thus reducing MTCT of HIV-1.
| Original language | English |
|---|---|
| Pages (from-to) | 211-217 |
| Number of pages | 7 |
| Journal | Current HIV Research |
| Volume | 7 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2009 |
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Keywords
- Antiretroviral prophylaxis
- HIV-1
- IL-10 cytokine
- Mother-to-child transmission
- Placenta
- Zidovudine and nevirapine
ASJC Scopus subject areas
- Infectious Diseases
- Virology
Cite this
Higher placental anti-inflammatory IL-10 cytokine expression in HIV-1 infected women receiving longer zidovudine prophylaxis associated with nevirapine. / Pornprasert, Sakorn; Mary, Jean Yves; Faye, Albert; Leechanachai, Pannee; Limtrakul, Aram; Rugpao, Sungwal; Sirivatanapa, Pannee; Gomuthbutra, Vorapin; Matanasaravoot, Wanmanee; Le Cœur, Sophie; Lallemant, Marc; Barré-Sinoussi, Françoise; Menu, Elisabeth; Ngo-Giang-Huong, Nicole; Ayouba, Ahidjo; Chailert, Sanupong; Chaouat, Gérard; Derrien, Muriel; Dolcini, Guillermina; Eteki, Nicole; Kfutwah, Anfumbom Jude; Kouo, Odette; Lemen, Brigitte; Maldonado-Estrada, Juan; Nerrienet, Eric; Njinku, Bernadette; Scarlatti, Gabriella; Tejiokem, Mathurin; Téné, Gilbert.
In: Current HIV Research, Vol. 7, No. 2, 2009, p. 211-217.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Higher placental anti-inflammatory IL-10 cytokine expression in HIV-1 infected women receiving longer zidovudine prophylaxis associated with nevirapine
AU - Pornprasert, Sakorn
AU - Mary, Jean Yves
AU - Faye, Albert
AU - Leechanachai, Pannee
AU - Limtrakul, Aram
AU - Rugpao, Sungwal
AU - Sirivatanapa, Pannee
AU - Gomuthbutra, Vorapin
AU - Matanasaravoot, Wanmanee
AU - Le Cœur, Sophie
AU - Lallemant, Marc
AU - Barré-Sinoussi, Françoise
AU - Menu, Elisabeth
AU - Ngo-Giang-Huong, Nicole
AU - Ayouba, Ahidjo
AU - Chailert, Sanupong
AU - Chaouat, Gérard
AU - Derrien, Muriel
AU - Dolcini, Guillermina
AU - Eteki, Nicole
AU - Kfutwah, Anfumbom Jude
AU - Kouo, Odette
AU - Lemen, Brigitte
AU - Maldonado-Estrada, Juan
AU - Nerrienet, Eric
AU - Njinku, Bernadette
AU - Scarlatti, Gabriella
AU - Tejiokem, Mathurin
AU - Téné, Gilbert
PY - 2009
Y1 - 2009
N2 - Placental cytokine balance may be critical for the control of mother-to-child transmission (MTCT) of HIV. We assessed whether the type and duration of antiretrovirals used for prevention of HIV-1-MTCT modified the inflammatory cytokine profile. We investigated the levels of cytokine expression in the placentas of 61 HIV-1-infected women who received zidovudine (ZDV) plus single dose nevirapine (SD-NVP) or ZDV only for prevention of MTCT. Placentas of 38 HIV-1-uninfected women were included as controls. All placentas were obtained after vaginal delivery. Levels of mRNA and cytokine expression were quantified using real-time PCR and ELISA, respectively, in placental explants and 24-hour culture supernatants and analyzed in relation to the women's characteristics and the type and duration of antiretroviral prophylaxis. HIV-1-infected and uninfected women did not show any differences in the expression of placental cytokine secretion except for a trend toward lower TNF-10 mRNA levels in HIV-1-infected women. Within the HIV-1-infected group, women who were exposed to a long duration of ZDV (>72 days) or received SD-NVP less than 5h prior to delivery, more frequently expressed detectable levels of IL-10 in their placentas (32% versus 7% (p = 0.01) and 32% versus 5% (p = 0.02), respectively). No infant was found to be HIV-1-infected. Our results showed a normalization of the placental cytokine balance in HIV-1-infected women receiving antiretroviral prophylaxis. Furthermore, the type and duration of antiretroviral prophylaxis have an impact on the placental anti-inflammatory IL-10 expression level, which may contribute to controlling HIV replication at the placental level, thus reducing MTCT of HIV-1.
AB - Placental cytokine balance may be critical for the control of mother-to-child transmission (MTCT) of HIV. We assessed whether the type and duration of antiretrovirals used for prevention of HIV-1-MTCT modified the inflammatory cytokine profile. We investigated the levels of cytokine expression in the placentas of 61 HIV-1-infected women who received zidovudine (ZDV) plus single dose nevirapine (SD-NVP) or ZDV only for prevention of MTCT. Placentas of 38 HIV-1-uninfected women were included as controls. All placentas were obtained after vaginal delivery. Levels of mRNA and cytokine expression were quantified using real-time PCR and ELISA, respectively, in placental explants and 24-hour culture supernatants and analyzed in relation to the women's characteristics and the type and duration of antiretroviral prophylaxis. HIV-1-infected and uninfected women did not show any differences in the expression of placental cytokine secretion except for a trend toward lower TNF-10 mRNA levels in HIV-1-infected women. Within the HIV-1-infected group, women who were exposed to a long duration of ZDV (>72 days) or received SD-NVP less than 5h prior to delivery, more frequently expressed detectable levels of IL-10 in their placentas (32% versus 7% (p = 0.01) and 32% versus 5% (p = 0.02), respectively). No infant was found to be HIV-1-infected. Our results showed a normalization of the placental cytokine balance in HIV-1-infected women receiving antiretroviral prophylaxis. Furthermore, the type and duration of antiretroviral prophylaxis have an impact on the placental anti-inflammatory IL-10 expression level, which may contribute to controlling HIV replication at the placental level, thus reducing MTCT of HIV-1.
KW - Antiretroviral prophylaxis
KW - HIV-1
KW - IL-10 cytokine
KW - Mother-to-child transmission
KW - Placenta
KW - Zidovudine and nevirapine
UR - http://www.scopus.com/inward/record.url?scp=64749097199&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=64749097199&partnerID=8YFLogxK
U2 - 10.2174/157016209787581472
DO - 10.2174/157016209787581472
M3 - Article
C2 - 19275589
AN - SCOPUS:64749097199
VL - 7
SP - 211
EP - 217
JO - Current HIV Research
JF - Current HIV Research
SN - 1570-162X
IS - 2
ER -