Highly homologous T-cell receptor beta sequences support a common target for autoreactive T cells in most patients with paroxysmal nocturnal hemoglobinuria

Lucia Gargiulo, Sonia Lastraioli, Giannamaria Cerruti, Martina Serra, Fabrizio Loiacono, Simona Zupo, Lucio Luzzatto, Rosario Notaro

Research output: Contribution to journalArticle

Abstract

Deficiency of glycosylphosphatidylinositol (GPI)-anchored molecules on blood cells accounts for most features of paroxysmal nocturnal hemoglobinuria (PNH) but not for the expansion of PNH (GPI-) clone(s). A plausible model is that PNH clones expand by escaping negative selection exerted by autoreactive T cells against normal (GPI+) hematopoiesis. By a systematic analysis of T-cell receptor beta (TCR-β) clonotypes of the CD8+ CD57+ T-cell population, frequently deranged in PNH, we show recurrent clonotypes in PNH patients but not in healthy controls: 11 of 16 patients shared at least 1 of 5 clonotypes, and a set of closely related clonotypes was present in 9 patients. The presence of T-cell clones bearing a set of highly homologous TCR-β molecules in most patients with hemolytic PNH is consistent with an immune process driven by the same (or similar) antigen(s) - probably a nonpeptide antigen, because patients sharing clonotypes do not all share identical HLA alleles. These data confirm that CD8+ CD57+ T cells play a role in PNH pathogenesis and provide strong new support to the hypothesis that the expansion of the GPI- blood cell population in PNH is due to selective damage to normal hematopoiesis mediated by an autoimmune attack against a nonpeptide antigen(s) that could be the GPI anchor itself.

Original languageEnglish
Pages (from-to)5036-5042
Number of pages7
JournalBlood
Volume109
Issue number11
DOIs
Publication statusPublished - Jun 1 2007

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Paroxysmal Hemoglobinuria
Glycosylphosphatidylinositols
T-cells
T-Cell Antigen Receptor
T-Lymphocytes
Antigens
Blood
Bearings (structural)
Cells
Clone Cells
Hematopoiesis
Molecules
Blood Cells
Population
Alleles

ASJC Scopus subject areas

  • Hematology

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Highly homologous T-cell receptor beta sequences support a common target for autoreactive T cells in most patients with paroxysmal nocturnal hemoglobinuria. / Gargiulo, Lucia; Lastraioli, Sonia; Cerruti, Giannamaria; Serra, Martina; Loiacono, Fabrizio; Zupo, Simona; Luzzatto, Lucio; Notaro, Rosario.

In: Blood, Vol. 109, No. 11, 01.06.2007, p. 5036-5042.

Research output: Contribution to journalArticle

Gargiulo, Lucia ; Lastraioli, Sonia ; Cerruti, Giannamaria ; Serra, Martina ; Loiacono, Fabrizio ; Zupo, Simona ; Luzzatto, Lucio ; Notaro, Rosario. / Highly homologous T-cell receptor beta sequences support a common target for autoreactive T cells in most patients with paroxysmal nocturnal hemoglobinuria. In: Blood. 2007 ; Vol. 109, No. 11. pp. 5036-5042.
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