Highly repeated DNA sequences and systematics of malagasy primates

S. Crovella; D. Montagnon; Y. Rumpler

doi:10.1007/BF02437512

Highly repeated DNA sequences and systematics of malagasy primates

S. Crovella, D. Montagnon, Y. Rumpler

IRCCS pediatrico Burlo Garofolo

Research output: Contribution to journal › Article › peer-review

Abstract

We have analyzed highly repeated DNA sequences of Malagasy lemurs with restriction enzymes, Southern blotting and nucleotide sequencing to assess relatedness among repeated DNA fragments from different species. We have focused our studies on two prosimian families:Lemuridae and Cheirogaleidae. Our results have allowed us to draw the following conclusions: confirmation of the separation based on molecular biology data of Eulemur species from the Lemur catta/Hapalemur group; classification of Varecia in a genus clearly separated from the other Lemuridae genera; confirmation of the specific status of Hapalemur aureus; confirmation of cytogenetic data in favour of the subdivision of the family Cheirogaleidae into two subfamilies:Cheirogaleinae and Phanerinae. Finally a cladogram has been constructed by numbering all the highly repeated DNA fragments (obtained by Southern blotting) and analyzing the inferred systematic relationships between the Lemuridae and Cheirogaleidae species.

Original language	English
Pages (from-to)	35-44
Number of pages	10
Journal	Human Evolution
Volume	10
Issue number	1
DOIs	https://doi.org/10.1007/BF02437512
Publication status	Published - Jan 1995

Keywords

DNA sequencing
Highly repeated DNA
lemurs
restriction enzymes
southern blotting
systematics

ASJC Scopus subject areas

Genetics
Ecology, Evolution, Behavior and Systematics

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10.1007/BF02437512

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title = "Highly repeated DNA sequences and systematics of malagasy primates",

abstract = "We have analyzed highly repeated DNA sequences of Malagasy lemurs with restriction enzymes, Southern blotting and nucleotide sequencing to assess relatedness among repeated DNA fragments from different species. We have focused our studies on two prosimian families:Lemuridae and Cheirogaleidae. Our results have allowed us to draw the following conclusions: confirmation of the separation based on molecular biology data of Eulemur species from the Lemur catta/Hapalemur group; classification of Varecia in a genus clearly separated from the other Lemuridae genera; confirmation of the specific status of Hapalemur aureus; confirmation of cytogenetic data in favour of the subdivision of the family Cheirogaleidae into two subfamilies:Cheirogaleinae and Phanerinae. Finally a cladogram has been constructed by numbering all the highly repeated DNA fragments (obtained by Southern blotting) and analyzing the inferred systematic relationships between the Lemuridae and Cheirogaleidae species.",

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author = "S. Crovella and D. Montagnon and Y. Rumpler",

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T1 - Highly repeated DNA sequences and systematics of malagasy primates

AU - Crovella, S.

AU - Montagnon, D.

AU - Rumpler, Y.

PY - 1995/1

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N2 - We have analyzed highly repeated DNA sequences of Malagasy lemurs with restriction enzymes, Southern blotting and nucleotide sequencing to assess relatedness among repeated DNA fragments from different species. We have focused our studies on two prosimian families:Lemuridae and Cheirogaleidae. Our results have allowed us to draw the following conclusions: confirmation of the separation based on molecular biology data of Eulemur species from the Lemur catta/Hapalemur group; classification of Varecia in a genus clearly separated from the other Lemuridae genera; confirmation of the specific status of Hapalemur aureus; confirmation of cytogenetic data in favour of the subdivision of the family Cheirogaleidae into two subfamilies:Cheirogaleinae and Phanerinae. Finally a cladogram has been constructed by numbering all the highly repeated DNA fragments (obtained by Southern blotting) and analyzing the inferred systematic relationships between the Lemuridae and Cheirogaleidae species.

AB - We have analyzed highly repeated DNA sequences of Malagasy lemurs with restriction enzymes, Southern blotting and nucleotide sequencing to assess relatedness among repeated DNA fragments from different species. We have focused our studies on two prosimian families:Lemuridae and Cheirogaleidae. Our results have allowed us to draw the following conclusions: confirmation of the separation based on molecular biology data of Eulemur species from the Lemur catta/Hapalemur group; classification of Varecia in a genus clearly separated from the other Lemuridae genera; confirmation of the specific status of Hapalemur aureus; confirmation of cytogenetic data in favour of the subdivision of the family Cheirogaleidae into two subfamilies:Cheirogaleinae and Phanerinae. Finally a cladogram has been constructed by numbering all the highly repeated DNA fragments (obtained by Southern blotting) and analyzing the inferred systematic relationships between the Lemuridae and Cheirogaleidae species.

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KW - Highly repeated DNA

KW - lemurs

KW - restriction enzymes

KW - southern blotting

KW - systematics

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Highly repeated DNA sequences and systematics of malagasy primates

Abstract

Keywords

ASJC Scopus subject areas

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