Abstract
We have analyzed highly repeated DNA sequences of Malagasy lemurs with restriction enzymes, Southern blotting and nucleotide sequencing to assess relatedness among repeated DNA fragments from different species. We have focused our studies on two prosimian families:Lemuridae and Cheirogaleidae. Our results have allowed us to draw the following conclusions: confirmation of the separation based on molecular biology data of Eulemur species from the Lemur catta/Hapalemur group; classification of Varecia in a genus clearly separated from the other Lemuridae genera; confirmation of the specific status of Hapalemur aureus; confirmation of cytogenetic data in favour of the subdivision of the family Cheirogaleidae into two subfamilies:Cheirogaleinae and Phanerinae. Finally a cladogram has been constructed by numbering all the highly repeated DNA fragments (obtained by Southern blotting) and analyzing the inferred systematic relationships between the Lemuridae and Cheirogaleidae species.
| Original language | English |
|---|---|
| Pages (from-to) | 35-44 |
| Number of pages | 10 |
| Journal | Human Evolution |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1995 |
Keywords
- DNA sequencing
- Highly repeated DNA
- lemurs
- restriction enzymes
- southern blotting
- systematics
ASJC Scopus subject areas
- Genetics
- Ecology, Evolution, Behavior and Systematics