HisE11 and HisF8 Provide Bis-histidyl Heme Hexa-coordination in the Globin Domain of Geobacter sulfurreducens Globin-coupled Sensor

Alessandra Pesce, Liesbet Thijs, Marco Nardini, Filip Desmet, Lorenza Sisinni, Louise Gourlay, Alessandro Bolli, Massimiliano Coletta, Sabine Van Doorslaer, Xuehua Wan, Maqsudul Alam, Paolo Ascenzi, Luc Moens, Martino Bolognesi, Sylvia Dewilde

Research output: Contribution to journalArticle

Abstract

Among heme-based sensors, recent phylogenomic and sequence analyses have identified 34 globin coupled sensors (GCS), to which an aerotactic or gene-regulating function has been tentatively ascribed. Here, the structural and biochemical characterization of the globin domain of the GCS from Geobacter sulfurreducens (GsGCS162) is reported. A combination of X-ray crystallography (crystal structure at 1.5 Å resolution), UV-vis and resonance Raman spectroscopy reveals the ferric GsGCS162 as an example of bis-histidyl hexa-coordinated GCS. In contrast to the known hexa-coordinated globins, the distal heme-coordination in ferric GsGCS162 is provided by a His residue unexpectedly located at the E11 topological site. Furthermore, UV-vis and resonance Raman spectroscopy indicated that ferrous deoxygenated GsGCS162 is a penta-/hexa-coordinated mixture, and the heme hexa-to-penta-coordination transition does not represent a rate-limiting step for carbonylation kinetics. Lastly, electron paramagnetic resonance indicates that ferrous nitrosylated GsGCS162 is a penta-coordinated species, where the proximal HisF8-Fe bond is severed.

Original languageEnglish
Pages (from-to)246-260
Number of pages15
JournalJournal of Molecular Biology
Volume386
Issue number1
DOIs
Publication statusPublished - Feb 13 2009

Keywords

  • 3D structure
  • Geobacter sulfurreducens
  • globin coupled sensor
  • hexacoordination
  • ligand binding properties

ASJC Scopus subject areas

  • Molecular Biology

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    Pesce, A., Thijs, L., Nardini, M., Desmet, F., Sisinni, L., Gourlay, L., Bolli, A., Coletta, M., Van Doorslaer, S., Wan, X., Alam, M., Ascenzi, P., Moens, L., Bolognesi, M., & Dewilde, S. (2009). HisE11 and HisF8 Provide Bis-histidyl Heme Hexa-coordination in the Globin Domain of Geobacter sulfurreducens Globin-coupled Sensor. Journal of Molecular Biology, 386(1), 246-260. https://doi.org/10.1016/j.jmb.2008.12.023