Histological changes induced by Polyglycolic-Acid (PGA) scaffolds seeded with autologous adipose or muscle-derived stem cells when implanted on rabbit bladder

Joao Paulo Zambon, Letícia Siqueira De Sá Barretto, Ahy Nathally Sawaki E Nakamura, Silvio Duailibi, Kátia Leite, Renata S. Magalhaes, Giuseppe Orlando, Christina L. Ross, Andrea Peloso, Fernando G. Almeida

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Purpose: To evaluate the morphological and histological changes induced by PGA scaffold seeded with autologous adipose or muscle derived stem cells implanted on rabbit bladder wall. Material and Methods: Adipose derived stem cells (ADSC s) were obtained from the inguinal fat of eight rabbits and muscle derived stem cells (MDSC s) from the anterior tibial muscle of other eight rabbits. After culture and isolation, the cells were stained with Vybrant Red CM DiI and then implanted at third passage. Two PGA scaffolds were implanted on the bladder submucosa of each animal. On the right bladder side was implanted unseeded PGA scaffold while on the left side was implanted ADSC s or skeletal MDSC s seeded PGA scaffold. ADSC s were implanted in eight animals and MDSC in other eight animals. The animals were sacrificed at four and eight weeks. Histological evaluation was performed with Hematoxylin and Eosin, Masson's Trichrome and smooth muscle a-actin. Results: We observed a mild inflammatory response in all the three groups. Seeded scaffolds induced higher lymphocytes and lower polimorphonuclear migration than controls. Fibrosis was more pronounced in the control groups. Smooth muscle a-actin was positive only in ADSC and MDSC seeded scaffolds. At four and eight weeks ADCS s and skeletal MDSC s labeled cells were found at the implant sites. Conclusions: The implantation of PGA scaffolds seeded with ADSC and MDSC induced less fibrosis than control and smooth muscle regeneration.

Original languageEnglish
Pages (from-to)278-288
Number of pages11
JournalOrganogenesis
Volume10
Issue number2
DOIs
Publication statusPublished - Apr 1 2014

Fingerprint

Polyglycolic Acid
Stem cells
Scaffolds
Muscle
Urinary Bladder
Stem Cells
Rabbits
Muscles
Acids
Animals
Smooth Muscle
Skeletal Muscle
Actins
Fibrosis
Groin
Cell Separation
Hematoxylin
Eosine Yellowish-(YS)
Lymphocytes
Scaffolds (biology)

Keywords

  • Adipocyte derived stem cells
  • Muscle derived stem cells
  • PGA scaffold
  • Rabbit bladder

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology
  • Transplantation
  • Biomedical Engineering

Cite this

Zambon, J. P., De Sá Barretto, L. S., Sawaki E Nakamura, A. N., Duailibi, S., Leite, K., Magalhaes, R. S., ... Almeida, F. G. (2014). Histological changes induced by Polyglycolic-Acid (PGA) scaffolds seeded with autologous adipose or muscle-derived stem cells when implanted on rabbit bladder. Organogenesis, 10(2), 278-288. https://doi.org/10.4161/org.29058

Histological changes induced by Polyglycolic-Acid (PGA) scaffolds seeded with autologous adipose or muscle-derived stem cells when implanted on rabbit bladder. / Zambon, Joao Paulo; De Sá Barretto, Letícia Siqueira; Sawaki E Nakamura, Ahy Nathally; Duailibi, Silvio; Leite, Kátia; Magalhaes, Renata S.; Orlando, Giuseppe; Ross, Christina L.; Peloso, Andrea; Almeida, Fernando G.

In: Organogenesis, Vol. 10, No. 2, 01.04.2014, p. 278-288.

Research output: Contribution to journalArticle

Zambon, JP, De Sá Barretto, LS, Sawaki E Nakamura, AN, Duailibi, S, Leite, K, Magalhaes, RS, Orlando, G, Ross, CL, Peloso, A & Almeida, FG 2014, 'Histological changes induced by Polyglycolic-Acid (PGA) scaffolds seeded with autologous adipose or muscle-derived stem cells when implanted on rabbit bladder', Organogenesis, vol. 10, no. 2, pp. 278-288. https://doi.org/10.4161/org.29058
Zambon, Joao Paulo ; De Sá Barretto, Letícia Siqueira ; Sawaki E Nakamura, Ahy Nathally ; Duailibi, Silvio ; Leite, Kátia ; Magalhaes, Renata S. ; Orlando, Giuseppe ; Ross, Christina L. ; Peloso, Andrea ; Almeida, Fernando G. / Histological changes induced by Polyglycolic-Acid (PGA) scaffolds seeded with autologous adipose or muscle-derived stem cells when implanted on rabbit bladder. In: Organogenesis. 2014 ; Vol. 10, No. 2. pp. 278-288.
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abstract = "Purpose: To evaluate the morphological and histological changes induced by PGA scaffold seeded with autologous adipose or muscle derived stem cells implanted on rabbit bladder wall. Material and Methods: Adipose derived stem cells (ADSC s) were obtained from the inguinal fat of eight rabbits and muscle derived stem cells (MDSC s) from the anterior tibial muscle of other eight rabbits. After culture and isolation, the cells were stained with Vybrant Red CM DiI and then implanted at third passage. Two PGA scaffolds were implanted on the bladder submucosa of each animal. On the right bladder side was implanted unseeded PGA scaffold while on the left side was implanted ADSC s or skeletal MDSC s seeded PGA scaffold. ADSC s were implanted in eight animals and MDSC in other eight animals. The animals were sacrificed at four and eight weeks. Histological evaluation was performed with Hematoxylin and Eosin, Masson's Trichrome and smooth muscle a-actin. Results: We observed a mild inflammatory response in all the three groups. Seeded scaffolds induced higher lymphocytes and lower polimorphonuclear migration than controls. Fibrosis was more pronounced in the control groups. Smooth muscle a-actin was positive only in ADSC and MDSC seeded scaffolds. At four and eight weeks ADCS s and skeletal MDSC s labeled cells were found at the implant sites. Conclusions: The implantation of PGA scaffolds seeded with ADSC and MDSC induced less fibrosis than control and smooth muscle regeneration.",
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AU - Zambon, Joao Paulo

AU - De Sá Barretto, Letícia Siqueira

AU - Sawaki E Nakamura, Ahy Nathally

AU - Duailibi, Silvio

AU - Leite, Kátia

AU - Magalhaes, Renata S.

AU - Orlando, Giuseppe

AU - Ross, Christina L.

AU - Peloso, Andrea

AU - Almeida, Fernando G.

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N2 - Purpose: To evaluate the morphological and histological changes induced by PGA scaffold seeded with autologous adipose or muscle derived stem cells implanted on rabbit bladder wall. Material and Methods: Adipose derived stem cells (ADSC s) were obtained from the inguinal fat of eight rabbits and muscle derived stem cells (MDSC s) from the anterior tibial muscle of other eight rabbits. After culture and isolation, the cells were stained with Vybrant Red CM DiI and then implanted at third passage. Two PGA scaffolds were implanted on the bladder submucosa of each animal. On the right bladder side was implanted unseeded PGA scaffold while on the left side was implanted ADSC s or skeletal MDSC s seeded PGA scaffold. ADSC s were implanted in eight animals and MDSC in other eight animals. The animals were sacrificed at four and eight weeks. Histological evaluation was performed with Hematoxylin and Eosin, Masson's Trichrome and smooth muscle a-actin. Results: We observed a mild inflammatory response in all the three groups. Seeded scaffolds induced higher lymphocytes and lower polimorphonuclear migration than controls. Fibrosis was more pronounced in the control groups. Smooth muscle a-actin was positive only in ADSC and MDSC seeded scaffolds. At four and eight weeks ADCS s and skeletal MDSC s labeled cells were found at the implant sites. Conclusions: The implantation of PGA scaffolds seeded with ADSC and MDSC induced less fibrosis than control and smooth muscle regeneration.

AB - Purpose: To evaluate the morphological and histological changes induced by PGA scaffold seeded with autologous adipose or muscle derived stem cells implanted on rabbit bladder wall. Material and Methods: Adipose derived stem cells (ADSC s) were obtained from the inguinal fat of eight rabbits and muscle derived stem cells (MDSC s) from the anterior tibial muscle of other eight rabbits. After culture and isolation, the cells were stained with Vybrant Red CM DiI and then implanted at third passage. Two PGA scaffolds were implanted on the bladder submucosa of each animal. On the right bladder side was implanted unseeded PGA scaffold while on the left side was implanted ADSC s or skeletal MDSC s seeded PGA scaffold. ADSC s were implanted in eight animals and MDSC in other eight animals. The animals were sacrificed at four and eight weeks. Histological evaluation was performed with Hematoxylin and Eosin, Masson's Trichrome and smooth muscle a-actin. Results: We observed a mild inflammatory response in all the three groups. Seeded scaffolds induced higher lymphocytes and lower polimorphonuclear migration than controls. Fibrosis was more pronounced in the control groups. Smooth muscle a-actin was positive only in ADSC and MDSC seeded scaffolds. At four and eight weeks ADCS s and skeletal MDSC s labeled cells were found at the implant sites. Conclusions: The implantation of PGA scaffolds seeded with ADSC and MDSC induced less fibrosis than control and smooth muscle regeneration.

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