TY - JOUR
T1 - Histological ex vivo evaluation of peri-incisional thermal effect created by a new-generation CO2 superpulsed laser
AU - Palaia, G.
AU - Del Vecchio, A.
AU - Impellizzeri, A.
AU - Tenore, G.
AU - Visca, P.
AU - Libotte, F.
AU - Russo, C.
AU - Romeo, U.
PY - 2014
Y1 - 2014
N2 - The purpose of this study is the evaluation of the histological effects of a new-generation superpulsed CO2 laser through an "ex vivo" study. A CO2 ( = 10,600 nm) ultra-speed laser (SmartUS20D, DEKA, Florence, Italy) has been used at different parameters from 2 to 4 watt in Continuous Wave (CW) and Pulsed Wave (PW, 50 Hz) to obtain 30 samples from pig cadaver tongues. All the specimens have been subdivided into 6 groups (from A to F) and each group consisted of 5 samples. A final specimen has been taken by scalpel and used as control group. Histological analysis has been performed using an optical microscope (Leica DM 2000) at a magnification of ×40. Results showed that histological readability was optimal in all the samples. The thermal damage has been negligible in all the groups. Furthermore, the average of thermal damage was 0,095 mm in the epithelial, while it was 0.245 mm in the connective tissue. Statistical analysis using Graphpad Prism 5 software showed no significant differences among the groups. CO2 laser demonstrated a good surgical effectiveness provoking little peripheral damage onto the cut edges and allowing a safe histological diagnosis.
AB - The purpose of this study is the evaluation of the histological effects of a new-generation superpulsed CO2 laser through an "ex vivo" study. A CO2 ( = 10,600 nm) ultra-speed laser (SmartUS20D, DEKA, Florence, Italy) has been used at different parameters from 2 to 4 watt in Continuous Wave (CW) and Pulsed Wave (PW, 50 Hz) to obtain 30 samples from pig cadaver tongues. All the specimens have been subdivided into 6 groups (from A to F) and each group consisted of 5 samples. A final specimen has been taken by scalpel and used as control group. Histological analysis has been performed using an optical microscope (Leica DM 2000) at a magnification of ×40. Results showed that histological readability was optimal in all the samples. The thermal damage has been negligible in all the groups. Furthermore, the average of thermal damage was 0,095 mm in the epithelial, while it was 0.245 mm in the connective tissue. Statistical analysis using Graphpad Prism 5 software showed no significant differences among the groups. CO2 laser demonstrated a good surgical effectiveness provoking little peripheral damage onto the cut edges and allowing a safe histological diagnosis.
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U2 - 10.1155/2014/345685
DO - 10.1155/2014/345685
M3 - Article
C2 - 24719574
AN - SCOPUS:84896946359
VL - 2014
JO - The Scientific World Journal
JF - The Scientific World Journal
SN - 2356-6140
M1 - 345685
ER -