Histological ex vivo evaluation of peri-incisional thermal effect created by a new-generation CO2 superpulsed laser

G. Palaia, A. Del Vecchio, A. Impellizzeri, G. Tenore, P. Visca, F. Libotte, C. Russo, U. Romeo

Research output: Contribution to journalArticlepeer-review

Abstract

The purpose of this study is the evaluation of the histological effects of a new-generation superpulsed CO2 laser through an "ex vivo" study. A CO2 ( = 10,600 nm) ultra-speed laser (SmartUS20D, DEKA, Florence, Italy) has been used at different parameters from 2 to 4 watt in Continuous Wave (CW) and Pulsed Wave (PW, 50 Hz) to obtain 30 samples from pig cadaver tongues. All the specimens have been subdivided into 6 groups (from A to F) and each group consisted of 5 samples. A final specimen has been taken by scalpel and used as control group. Histological analysis has been performed using an optical microscope (Leica DM 2000) at a magnification of ×40. Results showed that histological readability was optimal in all the samples. The thermal damage has been negligible in all the groups. Furthermore, the average of thermal damage was 0,095 mm in the epithelial, while it was 0.245 mm in the connective tissue. Statistical analysis using Graphpad Prism 5 software showed no significant differences among the groups. CO2 laser demonstrated a good surgical effectiveness provoking little peripheral damage onto the cut edges and allowing a safe histological diagnosis.

Original languageEnglish
Article number345685
JournalTheScientificWorldJournal
Volume2014
DOIs
Publication statusPublished - 2014

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Fingerprint Dive into the research topics of 'Histological ex vivo evaluation of peri-incisional thermal effect created by a new-generation CO2 superpulsed laser'. Together they form a unique fingerprint.

Cite this