Histological in vitro evaluation of the effects of Er: YAG laser on oral soft tissues

Umberto Romeo, Fabrizio Libotte, Gaspare Palaia, Alessandro Del Vecchio, Gianluca Tenore, Paolo Visca, Samir Nammour, Antonella Polimeni

Research output: Contribution to journalArticle

Abstract

In oral pathology, laser devices can provide important advantages, especially in the treatment of certain lesions. However, there is controversy about the use of some wavelengths in the analysis of suspected dysplastic or neoplastic lesions, raising doubt about the laser's suitability for use in biopsy procedures. In recent studies, the KTP and diode lasers have been used in biopsy procedures without histological artefacts. The aim of this in vitro study was to evaluate the exact extent of peripheral thermal damage to oral soft tissues caused by an Er:YAG laser (γ 2,940 nm) without water cooling. The study was performed on five swine cadaver tongues. Nine samples from each tongue were taken by the same operator using the Er:YAG laser with increasing energies (from 60 to 150 mJ) and fluencies (from 21 to 53 J/cm2). In addition to the laser samples, a specimen obtained using a scalpel was used as control. The samples were placed in 10% formalin solution and were examined by optical microscopy by two blinded pathologists who assigned a thermal damage score (from 0 to 3) to each sample. The Er:YAG laser produced less damage at 80 and 100 mJ and 28 and 35 J/cm2 (intermediate parameters). Although in some samples thermal damage was minimally visible, in all samples histological evaluation was clearly possible. The study demonstrated that the Er:YAG laser can be safely used in oral biopsy investigations while ensuring a successful histological evaluation, which is fundamental to correct clinical management.

Original languageEnglish
Pages (from-to)749-753
Number of pages5
JournalLasers in Medical Science
Volume27
Issue number4
DOIs
Publication statusPublished - Jul 2012

Fingerprint

Solid-State Lasers
Lasers
Hot Temperature
Biopsy
Tongue
Oral Pathology
Semiconductor Lasers
Cadaver
Artifacts
Formaldehyde
Microscopy
Swine
In Vitro Techniques
Equipment and Supplies
Water

Keywords

  • Er:YAG laser
  • Oral biopsy
  • Thermal damage

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

Romeo, U., Libotte, F., Palaia, G., Del Vecchio, A., Tenore, G., Visca, P., ... Polimeni, A. (2012). Histological in vitro evaluation of the effects of Er: YAG laser on oral soft tissues. Lasers in Medical Science, 27(4), 749-753. https://doi.org/10.1007/s10103-011-0969-z

Histological in vitro evaluation of the effects of Er : YAG laser on oral soft tissues. / Romeo, Umberto; Libotte, Fabrizio; Palaia, Gaspare; Del Vecchio, Alessandro; Tenore, Gianluca; Visca, Paolo; Nammour, Samir; Polimeni, Antonella.

In: Lasers in Medical Science, Vol. 27, No. 4, 07.2012, p. 749-753.

Research output: Contribution to journalArticle

Romeo, U, Libotte, F, Palaia, G, Del Vecchio, A, Tenore, G, Visca, P, Nammour, S & Polimeni, A 2012, 'Histological in vitro evaluation of the effects of Er: YAG laser on oral soft tissues', Lasers in Medical Science, vol. 27, no. 4, pp. 749-753. https://doi.org/10.1007/s10103-011-0969-z
Romeo, Umberto ; Libotte, Fabrizio ; Palaia, Gaspare ; Del Vecchio, Alessandro ; Tenore, Gianluca ; Visca, Paolo ; Nammour, Samir ; Polimeni, Antonella. / Histological in vitro evaluation of the effects of Er : YAG laser on oral soft tissues. In: Lasers in Medical Science. 2012 ; Vol. 27, No. 4. pp. 749-753.
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