Histomorphometric and microhardness assessments of sheep cortical bone surrounding titanium implants with different surface treatments

G. Giavaresi, M. Fini, A. Cigada, R. Chiesa, G. Rondelli, L. Rimondini, N. Nicoli Aldini, L. Martini, R. Giardino

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Several factors influence the healing process and the long-term mechanical stability of cementless fixed implants, such as bone remodeling and mineralization processes. Histomorphometric and bone hardness measurements were taken in implants inserted in sheep femoral cortical bone at different times to compare the in vivo osseointegration of titanium screws (diam.; 3.5 × 7 mm length) with the following surface treatments: machined (Ti-MA); acid-etched (Ti-HF); HA vacuum plasma spray (Ti-HA); and Ca-P anodization followed by a hydrothermal treatment (Ti-AM/HA). Ti-MA and Ti-AM/HA implants presented the lowest (Ra = 0.20 ± 0.01 μm) and highest (Ra = 1.97 ± 0.64 μm) significant (p <0.0005) roughness value, respectively. Bone-to-implant contact of Ti-HF was lower than that of the other surface treatments at both experimetal times (8 weeks: -20%, ns; 12 weeks: -30%, p <0.01). Significant differences in MAR NIAR (mineral apposition rate) were also found between the different experimental times for Ti-MA (115%, p <0.01) and Ti-HF (57%, p <0.01), demonstrating that bone growth had slowed inside the screw threads of Ti-HA and Ti-AM/HA after 12 weeks. No bone microhardness changes in preexisting host bone were found, while Ti-MA showed the lowest value for the inner thread area at 8 weeks (HV200 μm = 49.8 ± 3.8 HV). These findings confirm that osseointegration may be accelerated by adequate surface roughness and bioactive ceramic coating such as Ca-P anodization followed by a hydrothermal treatment, which enhance bone interlocking and mineralization.

Original languageEnglish
Pages (from-to)112-120
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume67
Issue number1
Publication statusPublished - Oct 1 2003

Fingerprint

Titanium
Microhardness
Surface treatment
Sheep
Bone
Osseointegration
Bone and Bones
Physiologic Calcification
Bone Remodeling
Bone Development
Hardness
Ceramics
Vacuum
Thigh
Minerals
Surface roughness
Screw threads
Acids
Ceramic coatings
Cortical Bone

Keywords

  • Histomorphometry
  • Microhardness
  • Sheep
  • Surface treatment
  • Titanium

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Histomorphometric and microhardness assessments of sheep cortical bone surrounding titanium implants with different surface treatments. / Giavaresi, G.; Fini, M.; Cigada, A.; Chiesa, R.; Rondelli, G.; Rimondini, L.; Aldini, N. Nicoli; Martini, L.; Giardino, R.

In: Journal of Biomedical Materials Research - Part A, Vol. 67, No. 1, 01.10.2003, p. 112-120.

Research output: Contribution to journalArticle

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abstract = "Several factors influence the healing process and the long-term mechanical stability of cementless fixed implants, such as bone remodeling and mineralization processes. Histomorphometric and bone hardness measurements were taken in implants inserted in sheep femoral cortical bone at different times to compare the in vivo osseointegration of titanium screws (diam.; 3.5 × 7 mm length) with the following surface treatments: machined (Ti-MA); acid-etched (Ti-HF); HA vacuum plasma spray (Ti-HA); and Ca-P anodization followed by a hydrothermal treatment (Ti-AM/HA). Ti-MA and Ti-AM/HA implants presented the lowest (Ra = 0.20 ± 0.01 μm) and highest (Ra = 1.97 ± 0.64 μm) significant (p <0.0005) roughness value, respectively. Bone-to-implant contact of Ti-HF was lower than that of the other surface treatments at both experimetal times (8 weeks: -20{\%}, ns; 12 weeks: -30{\%}, p <0.01). Significant differences in MAR NIAR (mineral apposition rate) were also found between the different experimental times for Ti-MA (115{\%}, p <0.01) and Ti-HF (57{\%}, p <0.01), demonstrating that bone growth had slowed inside the screw threads of Ti-HA and Ti-AM/HA after 12 weeks. No bone microhardness changes in preexisting host bone were found, while Ti-MA showed the lowest value for the inner thread area at 8 weeks (HV200 μm = 49.8 ± 3.8 HV). These findings confirm that osseointegration may be accelerated by adequate surface roughness and bioactive ceramic coating such as Ca-P anodization followed by a hydrothermal treatment, which enhance bone interlocking and mineralization.",
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