Histone deacetylase 1 inactivation by an adenovirus early gene product

Susanna Chiocca, Vladislav Kurtev, Riccardo Colombo, Roberto Boggio, M. Teresa Sciurpi, Gerald Brosch, Christian Seiser, Giulio F. Draetta, Matt Cotten

Research output: Contribution to journalArticle

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Abstract

Gam1 is an early gene product of the avian adenovirus CELO and is essential for viral replication [1]. Gam1 has no homology to any known proteins; however, its early expression and nuclear localization suggest that the protein functions to influence transcription in the infected cell. A determinant of eukaryotic gene expression is the acetylation state of chromosomal histones and other nuclear proteins [2]. We find that Gam1 expression increases the level of transcription from a variety of eukaryotic promoters, similar to the effect of treating cells with the histone deacetylase (HDAC) inhibitor trichostatin A (TSA [3]). We show that Gam1 can effectively inhibit histone deacetylation by HDAC1 and that Gam1 binds to HDAC1 both in vitro and in vivo. A CELO virus lacking Gam1 (CELOdG) is replication defective [1], but the defect can be overcome by either expressing an interfering HDAC1 mutant or by treating infected cells with TSA. The identification of a viral early gene product having the specific function of binding and inactivating HDAC suggests that deacetylase complexes play an important role in limiting early gene expression from invading viruses.

Original languageEnglish
Pages (from-to)594-598
Number of pages5
JournalCurrent Biology
Volume12
Issue number7
DOIs
Publication statusPublished - Apr 2 2002

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Histone Deacetylase 1
histone deacetylase
Adenoviridae
Transcription
Viruses
Gene expression
Histones
trichostatin A
inactivation
Genes
histones
Acetylation
Histone Deacetylase Inhibitors
Histone Deacetylases
Fowl adenovirus A
Aviadenovirus
transcription (genetics)
Nuclear Proteins
Gene Expression
gene expression

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Chiocca, S., Kurtev, V., Colombo, R., Boggio, R., Teresa Sciurpi, M., Brosch, G., ... Cotten, M. (2002). Histone deacetylase 1 inactivation by an adenovirus early gene product. Current Biology, 12(7), 594-598. https://doi.org/10.1016/S0960-9822(02)00720-0

Histone deacetylase 1 inactivation by an adenovirus early gene product. / Chiocca, Susanna; Kurtev, Vladislav; Colombo, Riccardo; Boggio, Roberto; Teresa Sciurpi, M.; Brosch, Gerald; Seiser, Christian; Draetta, Giulio F.; Cotten, Matt.

In: Current Biology, Vol. 12, No. 7, 02.04.2002, p. 594-598.

Research output: Contribution to journalArticle

Chiocca, S, Kurtev, V, Colombo, R, Boggio, R, Teresa Sciurpi, M, Brosch, G, Seiser, C, Draetta, GF & Cotten, M 2002, 'Histone deacetylase 1 inactivation by an adenovirus early gene product', Current Biology, vol. 12, no. 7, pp. 594-598. https://doi.org/10.1016/S0960-9822(02)00720-0
Chiocca S, Kurtev V, Colombo R, Boggio R, Teresa Sciurpi M, Brosch G et al. Histone deacetylase 1 inactivation by an adenovirus early gene product. Current Biology. 2002 Apr 2;12(7):594-598. https://doi.org/10.1016/S0960-9822(02)00720-0
Chiocca, Susanna ; Kurtev, Vladislav ; Colombo, Riccardo ; Boggio, Roberto ; Teresa Sciurpi, M. ; Brosch, Gerald ; Seiser, Christian ; Draetta, Giulio F. ; Cotten, Matt. / Histone deacetylase 1 inactivation by an adenovirus early gene product. In: Current Biology. 2002 ; Vol. 12, No. 7. pp. 594-598.
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