Histopathological effects induced by paraquat during Xenopus laevis primary myogenesis

P. Mantecca, S. Panseri, R. Bacchetta, C. Vismara, G. Vailati, M. Camatini

Research output: Contribution to journalArticle

Abstract

The oxidative agent paraquat induced tail abnormalities during Xenopus laevis development. Specimens exposed from blastula to the tadpole stage revealed pear-shaped myocytes and irregular intersomitic boundaries. The histological feature of the axial musculature was evaluated in embryos sampled at significant stages of the primary myogenesis. During the somitogenesis PQ-treated embryos showed normal appearing myotomes, but reduced PAS activity in the post-rotating myotomal cells, and myoblasts with slight vacuolations. Once etched from the vitelline envelope, embryos showed severely altered myoblasts with irregular cellular apexes, heavy sarcoplasmic vacuolations, pyknotic nuclei and disorganizing intersomitic boundaries. Myotomes with many necrotic myocytes containing disorganized contractile material and heavily malformed intersomitic boundaries characterized the late myogenic stages. Our results evidence the heaviest PQ histopathological effects to affect myogenesis of post-etched embryos, suggesting a possible linkage between the swimming activity and the oxidative damage to muscle tissue.

Original languageEnglish
Pages (from-to)209-217
Number of pages9
JournalTissue and Cell
Volume38
Issue number3
DOIs
Publication statusPublished - Jun 2006

Fingerprint

Paraquat
Muscle Development
muscle development
paraquat
Xenopus laevis
embryo (animal)
Embryonic Structures
myoblasts
Myoblasts
myocytes
Muscle Cells
Blastula
Pyrus
tadpoles
muscle tissues
pears
Larva
Tail
tail
Muscles

Keywords

  • Histopathology
  • Myogenesis
  • Myopathies
  • Oxidative stress
  • Paraquat
  • X. laevis

ASJC Scopus subject areas

  • Insect Science
  • Cell Biology

Cite this

Mantecca, P., Panseri, S., Bacchetta, R., Vismara, C., Vailati, G., & Camatini, M. (2006). Histopathological effects induced by paraquat during Xenopus laevis primary myogenesis. Tissue and Cell, 38(3), 209-217. https://doi.org/10.1016/j.tice.2006.03.002

Histopathological effects induced by paraquat during Xenopus laevis primary myogenesis. / Mantecca, P.; Panseri, S.; Bacchetta, R.; Vismara, C.; Vailati, G.; Camatini, M.

In: Tissue and Cell, Vol. 38, No. 3, 06.2006, p. 209-217.

Research output: Contribution to journalArticle

Mantecca, P, Panseri, S, Bacchetta, R, Vismara, C, Vailati, G & Camatini, M 2006, 'Histopathological effects induced by paraquat during Xenopus laevis primary myogenesis', Tissue and Cell, vol. 38, no. 3, pp. 209-217. https://doi.org/10.1016/j.tice.2006.03.002
Mantecca P, Panseri S, Bacchetta R, Vismara C, Vailati G, Camatini M. Histopathological effects induced by paraquat during Xenopus laevis primary myogenesis. Tissue and Cell. 2006 Jun;38(3):209-217. https://doi.org/10.1016/j.tice.2006.03.002
Mantecca, P. ; Panseri, S. ; Bacchetta, R. ; Vismara, C. ; Vailati, G. ; Camatini, M. / Histopathological effects induced by paraquat during Xenopus laevis primary myogenesis. In: Tissue and Cell. 2006 ; Vol. 38, No. 3. pp. 209-217.
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