Histopathological evaluation of retinal damage during intraocular hypertension in rabbit: Involvement of ganglion cells and Nerve Fiber Layer

Gianluca Manni, Alessandro Lambiase, Marco Centofanti, Eleonora Mattei, Alessandra De Gregorio, Luigi Aloe, Giuseppe De Feo

Research output: Contribution to journalArticle

Abstract

• Background: Current clinical tests do not detect glaucomatous signs until the onset of substantial retinal damage. Therefore animal models are required to investigate the very early histopathological alterations in glaucoma disease. We used an experimental model of intraocular hypertension to compare early changes in retinal ganglion cell (RGC) density with the thickness of the nerve fiber layer (NFL). • Methods: Methylcellulose 2% was injected into the anterior chamber of 18 eyes of 18 New Zealand albino rabbits. Intraocular pressure was measured 6 h after the injection and thenceforth once a day using a Shiötz tonometer. Histopathological analysis was performed on days 4, 10, and 15 following the induction of hypertension (six eyes for each group). Sections from the upper temporal retina were stained with hematoxylin-eosin and immunohistochemically using a polyclonal antibody PGP 9.5 to identify RGC. An image analysis system was used to evaluate the RGC and the thickness of the NFL. • Results: We observed a significant increase in intraocular pressure until the end of the experiment. Histological analysis showed, after 10 days of ocular hypertension, a significant decrease in RGC density (P

Original languageEnglish
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume234
Issue numberSUPPL. 1
Publication statusPublished - 1996

ASJC Scopus subject areas

  • Ophthalmology

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