HIV-1-derived lentiviral vectors directly activate plasmacytoid dendritic cells, which in turn induce the maturation of myeloid dendritic cells

Maura Rossetti; Silvia Gregori; Ehud Hauben; Brian D. Brown; Lucia Sergi Sergi; Luigi Naldini; Maria Grazia Roncarolo

doi:10.1089/hum.2010.085

HIV-1-derived lentiviral vectors directly activate plasmacytoid dendritic cells, which in turn induce the maturation of myeloid dendritic cells

Maura Rossetti, Silvia Gregori, Ehud Hauben, Brian D. Brown, Lucia Sergi Sergi, Luigi Naldini, Maria Grazia Roncarolo

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

Lentiviral vectors (LV) can induce type I interferon (IFN I) production from murine plasmacytoid dendritic cells (pDC), but not myeloid (my)DC. Here, we investigated whether this mechanism is conserved in human DC. MyDC and pDC were isolated from peripheral blood and transduced with increasing vector concentrations. Compared with in vitro differentiated monocyte-derived DC, the transduction efficiency of peripheral blood DC was low (ranging from

Original language	English
Pages (from-to)	177-188
Number of pages	12
Journal	Human Gene Therapy
Volume	22
Issue number	2
DOIs	https://doi.org/10.1089/hum.2010.085
Publication status	Published - Feb 1 2011

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Genetics

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HIV-1-derived lentiviral vectors directly activate plasmacytoid dendritic cells, which in turn induce the maturation of myeloid dendritic cells. / Rossetti, Maura; Gregori, Silvia; Hauben, Ehud; Brown, Brian D.; Sergi, Lucia Sergi; Naldini, Luigi; Roncarolo, Maria Grazia.

In: Human Gene Therapy, Vol. 22, No. 2, 01.02.2011, p. 177-188.

Research output: Contribution to journal › Article › peer-review

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