HIV-1-derived lentiviral vectors directly activate plasmacytoid dendritic cells, which in turn induce the maturation of myeloid dendritic cells

Maura Rossetti; Silvia Gregori; Ehud Hauben; Brian D. Brown; Lucia Sergi Sergi; Luigi Naldini; Maria Grazia Roncarolo

doi:10.1089/hum.2010.085

HIV-1-derived lentiviral vectors directly activate plasmacytoid dendritic cells, which in turn induce the maturation of myeloid dendritic cells

Maura Rossetti, Silvia Gregori, Ehud Hauben, Brian D. Brown, Lucia Sergi Sergi, Luigi Naldini, Maria Grazia Roncarolo

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

Lentiviral vectors (LV) can induce type I interferon (IFN I) production from murine plasmacytoid dendritic cells (pDC), but not myeloid (my)DC. Here, we investigated whether this mechanism is conserved in human DC. MyDC and pDC were isolated from peripheral blood and transduced with increasing vector concentrations. Compared with in vitro differentiated monocyte-derived DC, the transduction efficiency of peripheral blood DC was low (ranging from

Original language	English
Pages (from-to)	177-188
Number of pages	12
Journal	Human Gene Therapy
Volume	22
Issue number	2
DOIs	https://doi.org/10.1089/hum.2010.085
Publication status	Published - Feb 1 2011

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Genetics

Access to Document

10.1089/hum.2010.085

Cite this

HIV-1-derived lentiviral vectors directly activate plasmacytoid dendritic cells, which in turn induce the maturation of myeloid dendritic cells. / Rossetti, Maura; Gregori, Silvia; Hauben, Ehud; Brown, Brian D.; Sergi, Lucia Sergi; Naldini, Luigi; Roncarolo, Maria Grazia.

In: Human Gene Therapy, Vol. 22, No. 2, 01.02.2011, p. 177-188.

Research output: Contribution to journal › Article › peer-review

@article{4e977b8ef3684287af0410240faa67dd,

title = "HIV-1-derived lentiviral vectors directly activate plasmacytoid dendritic cells, which in turn induce the maturation of myeloid dendritic cells",

abstract = "Lentiviral vectors (LV) can induce type I interferon (IFN I) production from murine plasmacytoid dendritic cells (pDC), but not myeloid (my)DC. Here, we investigated whether this mechanism is conserved in human DC. MyDC and pDC were isolated from peripheral blood and transduced with increasing vector concentrations. Compared with in vitro differentiated monocyte-derived DC, the transduction efficiency of peripheral blood DC was low (ranging from ",

author = "Maura Rossetti and Silvia Gregori and Ehud Hauben and Brown, {Brian D.} and Sergi, {Lucia Sergi} and Luigi Naldini and Roncarolo, {Maria Grazia}",

year = "2011",

month = feb,

day = "1",

doi = "10.1089/hum.2010.085",

language = "English",

volume = "22",

pages = "177--188",

journal = "Human Gene Therapy",

issn = "1043-0342",

publisher = "Mary Ann Liebert Inc.",

number = "2",

}

TY - JOUR

T1 - HIV-1-derived lentiviral vectors directly activate plasmacytoid dendritic cells, which in turn induce the maturation of myeloid dendritic cells

AU - Rossetti, Maura

AU - Gregori, Silvia

AU - Hauben, Ehud

AU - Brown, Brian D.

AU - Sergi, Lucia Sergi

AU - Naldini, Luigi

AU - Roncarolo, Maria Grazia

PY - 2011/2/1

Y1 - 2011/2/1

N2 - Lentiviral vectors (LV) can induce type I interferon (IFN I) production from murine plasmacytoid dendritic cells (pDC), but not myeloid (my)DC. Here, we investigated whether this mechanism is conserved in human DC. MyDC and pDC were isolated from peripheral blood and transduced with increasing vector concentrations. Compared with in vitro differentiated monocyte-derived DC, the transduction efficiency of peripheral blood DC was low (ranging from

AB - Lentiviral vectors (LV) can induce type I interferon (IFN I) production from murine plasmacytoid dendritic cells (pDC), but not myeloid (my)DC. Here, we investigated whether this mechanism is conserved in human DC. MyDC and pDC were isolated from peripheral blood and transduced with increasing vector concentrations. Compared with in vitro differentiated monocyte-derived DC, the transduction efficiency of peripheral blood DC was low (ranging from

UR - http://www.scopus.com/inward/record.url?scp=79951840580&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79951840580&partnerID=8YFLogxK

U2 - 10.1089/hum.2010.085

DO - 10.1089/hum.2010.085

M3 - Article

C2 - 20825284

AN - SCOPUS:79951840580

VL - 22

SP - 177

EP - 188

JO - Human Gene Therapy

JF - Human Gene Therapy

SN - 1043-0342

IS - 2

ER -

HIV-1-derived lentiviral vectors directly activate plasmacytoid dendritic cells, which in turn induce the maturation of myeloid dendritic cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this