TY - JOUR
T1 - HIV-1 gp120-dependent induction of apoptosis in antigen-specific human T cell clones is characterized by 'tissue' transglutaminase expression and prevented by cyclosporin A
AU - Amendola, Alessandra
AU - Lombardi, Giovanna
AU - Oliverio, Serafina
AU - Colizzi, Vittorio
AU - Piacentini, Mauro
PY - 1994/2/21
Y1 - 1994/2/21
N2 - We investigated the effect of cyclosporin (CsA) on HIV-gpl20-dependent induction of cell death by apoptosis of human T cell clones specific for influenza virus haemagglutinin and restricted by HLA-DR1. Preincubation of the clones with gp120 induced a large inhibition of their proliferation which was paralleled by the induction of apoptosis. Exposure to the specific antigen alone was able to trigger apoptosis in a significant fraction of cells, this effect was potentiated by pretreatment with gp120. Apoptosis was characterized by the typical morphological changes and by the expression of 'tissue' Transglutaminase (tTG), one of the few characterized effector elements of programmed cell death. Interestingly, the tTG protein induction was detectable within the first 24 hours following the gp120 treatment and preceded the appearance of the typical apoptotic phenotype. Noteworthy, CsA treatment prevented the gp120-dependent induction ofapoptosis by blocking the activation of the Ca2+-dependent effector elements such as tTG.
AB - We investigated the effect of cyclosporin (CsA) on HIV-gpl20-dependent induction of cell death by apoptosis of human T cell clones specific for influenza virus haemagglutinin and restricted by HLA-DR1. Preincubation of the clones with gp120 induced a large inhibition of their proliferation which was paralleled by the induction of apoptosis. Exposure to the specific antigen alone was able to trigger apoptosis in a significant fraction of cells, this effect was potentiated by pretreatment with gp120. Apoptosis was characterized by the typical morphological changes and by the expression of 'tissue' Transglutaminase (tTG), one of the few characterized effector elements of programmed cell death. Interestingly, the tTG protein induction was detectable within the first 24 hours following the gp120 treatment and preceded the appearance of the typical apoptotic phenotype. Noteworthy, CsA treatment prevented the gp120-dependent induction ofapoptosis by blocking the activation of the Ca2+-dependent effector elements such as tTG.
KW - AIDS
KW - Immune suppresive agent
KW - Protein cross-linking
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U2 - 10.1016/0014-5793(94)80427-3
DO - 10.1016/0014-5793(94)80427-3
M3 - Article
C2 - 7906657
AN - SCOPUS:0028181091
VL - 339
SP - 258
EP - 264
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -