HIV-1 gp120-dependent induction of apoptosis in antigen-specific human T cell clones is characterized by 'tissue' transglutaminase expression and prevented by cyclosporin A

Alessandra Amendola, Giovanna Lombardi, Serafina Oliverio, Vittorio Colizzi, Mauro Piacentini

Research output: Contribution to journalArticlepeer-review

Abstract

We investigated the effect of cyclosporin (CsA) on HIV-gpl20-dependent induction of cell death by apoptosis of human T cell clones specific for influenza virus haemagglutinin and restricted by HLA-DR1. Preincubation of the clones with gp120 induced a large inhibition of their proliferation which was paralleled by the induction of apoptosis. Exposure to the specific antigen alone was able to trigger apoptosis in a significant fraction of cells, this effect was potentiated by pretreatment with gp120. Apoptosis was characterized by the typical morphological changes and by the expression of 'tissue' Transglutaminase (tTG), one of the few characterized effector elements of programmed cell death. Interestingly, the tTG protein induction was detectable within the first 24 hours following the gp120 treatment and preceded the appearance of the typical apoptotic phenotype. Noteworthy, CsA treatment prevented the gp120-dependent induction ofapoptosis by blocking the activation of the Ca2+-dependent effector elements such as tTG.

Original languageEnglish
Pages (from-to)258-264
Number of pages7
JournalFEBS Letters
Volume339
Issue number3
DOIs
Publication statusPublished - Feb 21 1994

Keywords

  • AIDS
  • Immune suppresive agent
  • Protein cross-linking

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'HIV-1 gp120-dependent induction of apoptosis in antigen-specific human T cell clones is characterized by 'tissue' transglutaminase expression and prevented by cyclosporin A'. Together they form a unique fingerprint.

Cite this