HIV-1 infection causes a down-regulation of genes involved in ribosome biogenesis

Claudia L. Kleinman, Margherita Doria, Elisa Orecchini, Erica Giuliani, Silvia Galardi, Nicolas De Jay, Alessandro Michienzi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

HIV-1 preferentially infects CD4+ T cells, causing fundamental changes that eventually lead to the release of new viral particles and cell death. To investigate in detail alterations in the transcriptome of the CD4+ T cells upon viral infection, we sequenced polyadenylated RNA isolated from Jurkat cells infected or not with HIV-1. We found a marked global alteration of gene expression following infection, with an overall trend toward induction of genes, indicating widespread modification of the host biology. Annotation and pathway analysis of the most deregulated genes showed that viral infection produces a down-regulation of genes associated with the nucleolus, in particular those implicated in regulating the different steps of ribosome biogenesis, such as ribosomal RNA (rRNA) transcription, pre-rRNA processing, and ribosome maturation. The impact of HIV-1 infection on genes involved in ribosome biogenesis was further validated in primary CD4+ T cells. Moreover, we provided evidence by Northern Blot experiments, that host pre-rRNA processing in Jurkat cells might be perturbed during HIV-1 infection, thus strengthening the hypothesis of a crosstalk between nucleolar functions and viral pathogenesis.

Original languageEnglish
Article numbere113908
JournalPLoS One
Volume9
Issue number12
DOIs
Publication statusPublished - Dec 2 2014

Fingerprint

Human immunodeficiency virus 1
ribosomes
Ribosomes
HIV Infections
T-cells
HIV-1
Down-Regulation
Genes
Jurkat Cells
Ribosomal RNA
Virus Diseases
T-Lymphocytes
infection
T-lymphocytes
ribosomal RNA
genes
Cell death
Transcription
Crosstalk
Processing

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

HIV-1 infection causes a down-regulation of genes involved in ribosome biogenesis. / Kleinman, Claudia L.; Doria, Margherita; Orecchini, Elisa; Giuliani, Erica; Galardi, Silvia; De Jay, Nicolas; Michienzi, Alessandro.

In: PLoS One, Vol. 9, No. 12, e113908, 02.12.2014.

Research output: Contribution to journalArticle

Kleinman, Claudia L. ; Doria, Margherita ; Orecchini, Elisa ; Giuliani, Erica ; Galardi, Silvia ; De Jay, Nicolas ; Michienzi, Alessandro. / HIV-1 infection causes a down-regulation of genes involved in ribosome biogenesis. In: PLoS One. 2014 ; Vol. 9, No. 12.
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