HIV-1 provirus detection by PCR in seronegative drug addicts

R. Novati, L. Ciaffi, T. S. Migone, T. Bini, G. Morsica, A. Ruggieri, A. Nicolosi, A. Lazzarin

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

One hundred and eleven whole blood samples from a cohort of HIV negative drug addicts with a high frequence of needle sharing were analyzed by polymerase chain reaction for HIV-1 DNA, to study the existence and the rate of silent HIV infection in this high risk category. DNA was extracted and amplified with SK 38-39 GAG primers of HIV. Hybridization of PCR products was carried out with a biotinilated probe (GAG SK 19), in a microplate Elisa system. Six out of 111 patients (5.4%) had a positive PCR result, confirmed over replicate experiments. Follow-up is until now available for two of them; both are still seronegative 6 and 14 months after the positive PCR result, respectively. None of the PCR positive patients had clinical symptoms or immunological abnormalities referable to HIV infection. One patient seroconverted 11 months after a negative PCR result. We conclude that silent HIV infection in this group of high risk patients seems more frequent than reported in other studies on similar cohorts of subjects at risk of HIV infection. Ongoing follow-up of PCR positive cases could clarify the clinical and virological outome of these patients. The use of microplate non radioactive hybridization system may allow a rapid and efficient detection of HIV-1 PCR products in large screening studies on high risk categories.

Original languageEnglish
Title of host publicationArchives of STD/HIV Research
Pages77-83
Number of pages7
Volume7
Edition2
Publication statusPublished - 1993

Fingerprint

Proviruses
Drug Users
HIV-1
Polymerase Chain Reaction
HIV Infections
HIV
Needle Sharing
DNA

ASJC Scopus subject areas

  • Dermatology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Novati, R., Ciaffi, L., Migone, T. S., Bini, T., Morsica, G., Ruggieri, A., ... Lazzarin, A. (1993). HIV-1 provirus detection by PCR in seronegative drug addicts. In Archives of STD/HIV Research (2 ed., Vol. 7, pp. 77-83)

HIV-1 provirus detection by PCR in seronegative drug addicts. / Novati, R.; Ciaffi, L.; Migone, T. S.; Bini, T.; Morsica, G.; Ruggieri, A.; Nicolosi, A.; Lazzarin, A.

Archives of STD/HIV Research. Vol. 7 2. ed. 1993. p. 77-83.

Research output: Chapter in Book/Report/Conference proceedingChapter

Novati, R, Ciaffi, L, Migone, TS, Bini, T, Morsica, G, Ruggieri, A, Nicolosi, A & Lazzarin, A 1993, HIV-1 provirus detection by PCR in seronegative drug addicts. in Archives of STD/HIV Research. 2 edn, vol. 7, pp. 77-83.
Novati R, Ciaffi L, Migone TS, Bini T, Morsica G, Ruggieri A et al. HIV-1 provirus detection by PCR in seronegative drug addicts. In Archives of STD/HIV Research. 2 ed. Vol. 7. 1993. p. 77-83
Novati, R. ; Ciaffi, L. ; Migone, T. S. ; Bini, T. ; Morsica, G. ; Ruggieri, A. ; Nicolosi, A. ; Lazzarin, A. / HIV-1 provirus detection by PCR in seronegative drug addicts. Archives of STD/HIV Research. Vol. 7 2. ed. 1993. pp. 77-83
@inbook{fa50ae30892d42bcbd51229e82afee08,
title = "HIV-1 provirus detection by PCR in seronegative drug addicts",
abstract = "One hundred and eleven whole blood samples from a cohort of HIV negative drug addicts with a high frequence of needle sharing were analyzed by polymerase chain reaction for HIV-1 DNA, to study the existence and the rate of silent HIV infection in this high risk category. DNA was extracted and amplified with SK 38-39 GAG primers of HIV. Hybridization of PCR products was carried out with a biotinilated probe (GAG SK 19), in a microplate Elisa system. Six out of 111 patients (5.4{\%}) had a positive PCR result, confirmed over replicate experiments. Follow-up is until now available for two of them; both are still seronegative 6 and 14 months after the positive PCR result, respectively. None of the PCR positive patients had clinical symptoms or immunological abnormalities referable to HIV infection. One patient seroconverted 11 months after a negative PCR result. We conclude that silent HIV infection in this group of high risk patients seems more frequent than reported in other studies on similar cohorts of subjects at risk of HIV infection. Ongoing follow-up of PCR positive cases could clarify the clinical and virological outome of these patients. The use of microplate non radioactive hybridization system may allow a rapid and efficient detection of HIV-1 PCR products in large screening studies on high risk categories.",
author = "R. Novati and L. Ciaffi and Migone, {T. S.} and T. Bini and G. Morsica and A. Ruggieri and A. Nicolosi and A. Lazzarin",
year = "1993",
language = "English",
volume = "7",
pages = "77--83",
booktitle = "Archives of STD/HIV Research",
edition = "2",

}

TY - CHAP

T1 - HIV-1 provirus detection by PCR in seronegative drug addicts

AU - Novati, R.

AU - Ciaffi, L.

AU - Migone, T. S.

AU - Bini, T.

AU - Morsica, G.

AU - Ruggieri, A.

AU - Nicolosi, A.

AU - Lazzarin, A.

PY - 1993

Y1 - 1993

N2 - One hundred and eleven whole blood samples from a cohort of HIV negative drug addicts with a high frequence of needle sharing were analyzed by polymerase chain reaction for HIV-1 DNA, to study the existence and the rate of silent HIV infection in this high risk category. DNA was extracted and amplified with SK 38-39 GAG primers of HIV. Hybridization of PCR products was carried out with a biotinilated probe (GAG SK 19), in a microplate Elisa system. Six out of 111 patients (5.4%) had a positive PCR result, confirmed over replicate experiments. Follow-up is until now available for two of them; both are still seronegative 6 and 14 months after the positive PCR result, respectively. None of the PCR positive patients had clinical symptoms or immunological abnormalities referable to HIV infection. One patient seroconverted 11 months after a negative PCR result. We conclude that silent HIV infection in this group of high risk patients seems more frequent than reported in other studies on similar cohorts of subjects at risk of HIV infection. Ongoing follow-up of PCR positive cases could clarify the clinical and virological outome of these patients. The use of microplate non radioactive hybridization system may allow a rapid and efficient detection of HIV-1 PCR products in large screening studies on high risk categories.

AB - One hundred and eleven whole blood samples from a cohort of HIV negative drug addicts with a high frequence of needle sharing were analyzed by polymerase chain reaction for HIV-1 DNA, to study the existence and the rate of silent HIV infection in this high risk category. DNA was extracted and amplified with SK 38-39 GAG primers of HIV. Hybridization of PCR products was carried out with a biotinilated probe (GAG SK 19), in a microplate Elisa system. Six out of 111 patients (5.4%) had a positive PCR result, confirmed over replicate experiments. Follow-up is until now available for two of them; both are still seronegative 6 and 14 months after the positive PCR result, respectively. None of the PCR positive patients had clinical symptoms or immunological abnormalities referable to HIV infection. One patient seroconverted 11 months after a negative PCR result. We conclude that silent HIV infection in this group of high risk patients seems more frequent than reported in other studies on similar cohorts of subjects at risk of HIV infection. Ongoing follow-up of PCR positive cases could clarify the clinical and virological outome of these patients. The use of microplate non radioactive hybridization system may allow a rapid and efficient detection of HIV-1 PCR products in large screening studies on high risk categories.

UR - http://www.scopus.com/inward/record.url?scp=0027153478&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027153478&partnerID=8YFLogxK

M3 - Chapter

VL - 7

SP - 77

EP - 83

BT - Archives of STD/HIV Research

ER -