HIV-1 tat protects CD4+ Jurkat T lymphoblastoid cells from apoptosis mediated by TNF-related apoptosis-inducing ligand

Davide Gibellini, Maria Carla Re, Cristina Ponti, Claudia Maldini, Claudio Celeghini, Alessandra Cappellini, Michele La Placa, Giorgio Zauli

Research output: Contribution to journalArticlepeer-review

Abstract

We have here investigated the effect of TNF-related apoptosis-inducing ligand (TRAIL), a new member of the TNF cytokine superfamily, on the survival of Jurkat lymphoblastoid cell lines stably transfected with plasmids expressing the wild-type or mutated (Cys22) human immunodeficiency virus type 1 (HIV-1) tat gene. Jurkat cells transfected with wild-type tat were resistant to TRAIL-mediated apoptosis, while Jurkat cells mock-transfected with the control plasmid or with a mutated nonfunctional tat cDNA were highly susceptible to TRAIL-mediated apoptosis. Also, pretreatment with low concentrations (10-100 ng/ml) of extracellular synthetic Tat protein partially protected Jurkat cells from TRAIL-mediated apoptosis. Taken together, these results demonstrated that endogenously expressed tat and, to a lesser extent, extracellular Tat block TRAIL-mediated apoptosis. Since it has been shown that primary lymphoid T cells purified from HIV-1-infected individuals are more susceptible than those purified from normal individuals to TRAIL-mediated apoptosis, our findings underscore a potentially important role of Tat in protecting HIV-1 infected cells from TRAIL-mediated apoptosis.

Original languageEnglish
Pages (from-to)89-99
Number of pages11
JournalCellular Immunology
Volume207
Issue number2
DOIs
Publication statusPublished - Feb 1 2001

Keywords

  • Apoptosis
  • HIV-1
  • Tat
  • TRAIL

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Fingerprint Dive into the research topics of 'HIV-1 tat protects CD4+ Jurkat T lymphoblastoid cells from apoptosis mediated by TNF-related apoptosis-inducing ligand'. Together they form a unique fingerprint.

Cite this