HIV avidity index performance using a modified fourth-generation immunoassay to detect recent HIV infections

B. Suligoi, V. Regine, M. Raimondo, A. Rodella, L. Terlenghi, A. Caruso, P. Bagnarelli, M.R. Capobianchi, N. Zanchetta, V. Ghisetti, C. Galli

Research output: Contribution to journalArticle

Abstract

Detecting recent HIV infections is important to evaluate incidence and monitor epidemic trends. We aimed to evaluate the diagnostic performance and accuracy of the avidity index (AI) for discriminating for recent HIV infections. We collected serum samples from HIV-1 positive individuals: A) with known date of infection (midpoint in time between last HIV-negative and first HIV-positive test); B) infected for >1 year. Samples were divided into two aliquots: one diluted with phosphate buffered saline (PBS) and the other with 1 M guanidine. Both aliquots were assayed by the Architect HIV Ag/Ab Combo 4th generation assay (Abbott). We compared AI found in recent (RI=
Original languageEnglish
Pages (from-to)2010-2019
Number of pages10
JournalClinical Chemistry and Laboratory Medicine
Volume55
Issue number12
DOIs
Publication statusPublished - 2017

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Keywords

  • avidity
  • enzyme immunoassay
  • epidemiology
  • HIV
  • incidence
  • recent infection
  • anti human immunodeficiency virus agent
  • antigen p24
  • Human immunodeficiency virus antibody
  • Human immunodeficiency virus antigen
  • virus RNA
  • adolescent
  • adult
  • aged
  • antiretroviral therapy
  • Article
  • CD4 lymphocyte count
  • cohort analysis
  • comparative study
  • controlled study
  • diagnostic accuracy
  • disease classification
  • female
  • human
  • Human immunodeficiency virus 1 infection
  • Human immunodeficiency virus avidity index immunoassay
  • Human immunodeficiency virus infected patient
  • immunoassay
  • major clinical study
  • male
  • middle aged
  • multicenter study
  • observational study
  • open study
  • priority journal
  • receiver operating characteristic
  • seroconversion
  • validation study
  • virus load
  • antibody affinity
  • blood
  • clinical trial
  • Human immunodeficiency virus
  • Human immunodeficiency virus infection
  • immunology
  • isolation and purification
  • young adult
  • Adolescent
  • Adult
  • Antibody Affinity
  • Cohort Studies
  • Female
  • HIV Infections
  • Humans
  • Immunoassay
  • Male
  • Middle Aged
  • Young Adult

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