HIV infection leads to differential expression of T-cell receptor Vβ genes in CD4+ and CD8+ T cells

Vida L. Hodara, Mahmood Jeddi-Tehrani, Johan Grunewald, Roland Andersson, Gabriella Scarlatti, Semih Esin, Viveca Holmberg, Osvaldo Libonatti, Hans Wigzell

Research output: Contribution to journalArticlepeer-review


Objective: To analyse variation in T-cell receptor (TCR) Vβ gene expression in T cells in HIV-infected individuals. Design: Because there are very few monoclonal antibodies available for studying TCR Vβ gene expression, we used polymerase chain reaction (PCR) to analyse the TCR Vβ repertoire in HIV-infected individuals using specific primers for 20 distinct families of TCR Vβ. Methods: Evaluation of TCR Vβ gene expression in peripheral blood from HIV-1-infected individuals [two in Centers for Disease Control (CDC) stage II, five in CDC stage III and four in CDC stage IV]. Complementary DNA was produced from CD4+ and CD8+ T cells, amplified by PCR and analysed after Southern blotting and hybridization with a Cβ-specific oligonucleotide probe. Results: Vβ gene expression was dramatically modified, especially in AIDS patients. The CD4+ T-cell subset showed both overexpression (Vβ2) and deletions or underexpression (Vβ9-Vβ20), whereas these gene segments were expressed normally in the CD8+ subset. Only Vβ 3 was deleted or underexpression in both CD4+ and CD8+ populations in AIDS patients. Conlusions: HIV-1 infection induces CD4+ T-cell deficiency, both in total numbers and by causing a paucity of select Vβ gene expression in this subset. In addition, the Vβ3 gene family was deleted or underexpressed was observed in both CD4+ and CD8+ T-cell subsets from patients in CDC stage IV . These results are compatible with changes in Vβ gene expression known to occur under the action of endogenous or exogenous superantigens.

Original languageEnglish
Pages (from-to)633-638
Number of pages6
JournalAIDS (London, England)
Issue number5
Publication statusPublished - May 1993


  • HIV
  • Polymerase chain reaction
  • Superantigens
  • T cells
  • T-cell receptor

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy


Dive into the research topics of 'HIV infection leads to differential expression of T-cell receptor Vβ genes in CD4+ and CD8+ T cells'. Together they form a unique fingerprint.

Cite this