HIV-1 RNA was detected by reverse transcription PCR (RT-PCR) on 64 replicate serum samples obtained at monthly intervals from 11 seropositive patients after the start of therapy with anti-retroviral agents, AZT or DDI. Forty-one sera (64% ) were positive for both GAG and POL primers. Fifty-three out of the 64 sera (82,8% ) were PCR positive with at least one primer pair. Eight patients were persistently RT-PCR positive; one had intermittent viremia; in two cases RT-PCR became negative during a three months period after the start of therapy. HIV p24 antigen (Ag) titers showed a transient decrease in 6 out of 9 patients. HIV-RNA was detected in all HIV p24 Ag positive samples and in 18/29 (62%) p24 Ag negative sera. HIV RNA was detected in all patients with a CD4+ cell count below 200/mm3, in 74% of cases with CD4+ cells between 200 and 400/mm3 and in 63% of patients with CD4+ cells above 400/mm3. Thus, HIV-RNA persists in the serum of most patients despite the start of AZT or DDI therapy. HIV-RNA detection by RT-PCR may be a valuable tool for virological monitoring in patients undergoing anti-retroviral therapy.
|Number of pages||5|
|Journal||Giornale di Malattie Infettive e Parassitarie|
|Publication status||Published - 1994|
ASJC Scopus subject areas