UTILIZZO DELLA DETERMINAZIONE DELL'HIV RNA SU SIERO MEDIANTE RT-PCR PER MONITORARE L'EFFICACIA DELLA TERAPIA ANTIRETROVIRALE

Translated title of the contribution: HIV RNA detection by RT-PCR as a valuable tool for virological monitoring of anti-retroviral therapy

A. Ruggieri, A. Chaput, R. Novati, T. Bini, A. D'Arminio Monforte, N. Zanchetta, A. Lazzarin, C. Brechot

Research output: Contribution to journalArticle

Abstract

HIV-1 RNA was detected by reverse transcription PCR (RT-PCR) on 64 replicate serum samples obtained at monthly intervals from 11 seropositive patients after the start of therapy with anti-retroviral agents, AZT or DDI. Forty-one sera (64% ) were positive for both GAG and POL primers. Fifty-three out of the 64 sera (82,8% ) were PCR positive with at least one primer pair. Eight patients were persistently RT-PCR positive; one had intermittent viremia; in two cases RT-PCR became negative during a three months period after the start of therapy. HIV p24 antigen (Ag) titers showed a transient decrease in 6 out of 9 patients. HIV-RNA was detected in all HIV p24 Ag positive samples and in 18/29 (62%) p24 Ag negative sera. HIV RNA was detected in all patients with a CD4+ cell count below 200/mm3, in 74% of cases with CD4+ cells between 200 and 400/mm3 and in 63% of patients with CD4+ cells above 400/mm3. Thus, HIV-RNA persists in the serum of most patients despite the start of AZT or DDI therapy. HIV-RNA detection by RT-PCR may be a valuable tool for virological monitoring in patients undergoing anti-retroviral therapy.

Translated title of the contributionHIV RNA detection by RT-PCR as a valuable tool for virological monitoring of anti-retroviral therapy
Original languageItalian
Pages (from-to)313-317
Number of pages5
JournalGiornale di Malattie Infettive e Parassitarie
Volume46
Issue number5
Publication statusPublished - 1994

ASJC Scopus subject areas

  • Parasitology

Fingerprint Dive into the research topics of 'HIV RNA detection by RT-PCR as a valuable tool for virological monitoring of anti-retroviral therapy'. Together they form a unique fingerprint.

  • Cite this

    Ruggieri, A., Chaput, A., Novati, R., Bini, T., D'Arminio Monforte, A., Zanchetta, N., Lazzarin, A., & Brechot, C. (1994). UTILIZZO DELLA DETERMINAZIONE DELL'HIV RNA SU SIERO MEDIANTE RT-PCR PER MONITORARE L'EFFICACIA DELLA TERAPIA ANTIRETROVIRALE. Giornale di Malattie Infettive e Parassitarie, 46(5), 313-317.