Abstract
A cohort of 39 vertically infected children (class N, A, B, and C of the CDC HIV classification for pediatric infection) was studied by virus isolation and non-syncytium inducing (NSI)/syncytium inducing (SI) HIV-1 phenotype evaluation. The HIV-1 isolates were recovered from PBMCs and the MT-2 cell line was used to perform the syncytium assay. HIV-1 could be isolated in 34 of 39 (87%) infected children, regardless of the clinical and immunological stage of the disease. Class N and A subjects harbored exclusively NSI strains, whereas the SI phenotype was detected in two of eight class B and five of nine class C patients. All of the SI variants were observed in severely CD4-depleted children (class 3 patients). The capability of pediatric HIV-1 isolates to induce a cytopathic effect is associated with the clinical status and the degree of CD4 depletion. These data suggest that the biological properties of HIV-1 isolates in children do not differ from those observed in adults, and that viral phenotype strictly correlates with disease progression in vertically infected children.
| Original language | English |
|---|---|
| Pages (from-to) | 1247-1253 |
| Number of pages | 7 |
| Journal | AIDS Research and Human Retroviruses |
| Volume | 12 |
| Issue number | 13 |
| Publication status | Published - Sep 1 1996 |
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ASJC Scopus subject areas
- Immunology
- Virology
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HIV type 1 phenotype correlates with the stage of infection in vertically infected children. / Balotta, Claudia; Viganò, Alessandra; Riva, Chiara; Colombo, M. Chiara; Salvaggio, Antonino; De Pasquale, M. Pia; Crupi, Lina; Papagno, Laura; Galli, Massimo; Moroni, Mauro; Principi, Nicola.
In: AIDS Research and Human Retroviruses, Vol. 12, No. 13, 01.09.1996, p. 1247-1253.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - HIV type 1 phenotype correlates with the stage of infection in vertically infected children
AU - Balotta, Claudia
AU - Viganò, Alessandra
AU - Riva, Chiara
AU - Colombo, M. Chiara
AU - Salvaggio, Antonino
AU - De Pasquale, M. Pia
AU - Crupi, Lina
AU - Papagno, Laura
AU - Galli, Massimo
AU - Moroni, Mauro
AU - Principi, Nicola
PY - 1996/9/1
Y1 - 1996/9/1
N2 - A cohort of 39 vertically infected children (class N, A, B, and C of the CDC HIV classification for pediatric infection) was studied by virus isolation and non-syncytium inducing (NSI)/syncytium inducing (SI) HIV-1 phenotype evaluation. The HIV-1 isolates were recovered from PBMCs and the MT-2 cell line was used to perform the syncytium assay. HIV-1 could be isolated in 34 of 39 (87%) infected children, regardless of the clinical and immunological stage of the disease. Class N and A subjects harbored exclusively NSI strains, whereas the SI phenotype was detected in two of eight class B and five of nine class C patients. All of the SI variants were observed in severely CD4-depleted children (class 3 patients). The capability of pediatric HIV-1 isolates to induce a cytopathic effect is associated with the clinical status and the degree of CD4 depletion. These data suggest that the biological properties of HIV-1 isolates in children do not differ from those observed in adults, and that viral phenotype strictly correlates with disease progression in vertically infected children.
AB - A cohort of 39 vertically infected children (class N, A, B, and C of the CDC HIV classification for pediatric infection) was studied by virus isolation and non-syncytium inducing (NSI)/syncytium inducing (SI) HIV-1 phenotype evaluation. The HIV-1 isolates were recovered from PBMCs and the MT-2 cell line was used to perform the syncytium assay. HIV-1 could be isolated in 34 of 39 (87%) infected children, regardless of the clinical and immunological stage of the disease. Class N and A subjects harbored exclusively NSI strains, whereas the SI phenotype was detected in two of eight class B and five of nine class C patients. All of the SI variants were observed in severely CD4-depleted children (class 3 patients). The capability of pediatric HIV-1 isolates to induce a cytopathic effect is associated with the clinical status and the degree of CD4 depletion. These data suggest that the biological properties of HIV-1 isolates in children do not differ from those observed in adults, and that viral phenotype strictly correlates with disease progression in vertically infected children.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029816985&partnerID=8YFLogxK
M3 - Article
C2 - 8870846
AN - SCOPUS:0029816985
VL - 12
SP - 1247
EP - 1253
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
SN - 0889-2229
IS - 13
ER -