HIV type 1 protease inhibitors enhance bone marrow progenitor cell activity in normal subjects and in HIV type 1-infected patients

Antonella Isgrò, Alessandro Aiuti, Ivano Mezzaroma, Luigi Ruco, Marcello Pinti, Andrea Cossarizza, Fernando Aiuti

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

HIV-1 protease inhibitors (PIs) may improve hematopoietic functions owing to their direct effects on bone marrow (BM) progenitor cells. In this study we investigated this hypothesis evaluating the effect of adding ritonavir (RTV) and indinavir (IND) on hematopoietic colony formation assays by colony-forming cell (CFC) and long-term culture-initiating cell (LTC-IC) assays, on apoptosis, on cytokine production and stromal cells, in subjects with HIV-1 infection, and in seronegative controls. After PI addition, CFC and LTC-IC assays in HIV-1-infected patients showed levels of colony growth significantly higher than those observed at baseline; the same PI activity on colony formation was observed in healthy subjects. No significant modifications on Fas, the membrane form of Fas (mFas) and Fas-ligand (FasL) expression, and on cytokine production were observed at BM level after the addition of PIs. At baseline, in HIV-1-infected patients, the majority of the stromal cells appeared as large and rounded, whereas after the addition of RTV or IND the stromal cells exhibited a "fibroblast-like" morphology and produced higher stem cell factor (SCF) and lower MIP-1α levels when compared with the stromal production without the addition of IND. RTV and IND increased colony growth of BM obtained either from HIV-1-infected patients or from normal individuals, in parallel with the normalization of functional and morphological characteristics of stromal cells.

Original languageEnglish
Pages (from-to)51-57
Number of pages7
JournalAIDS Research and Human Retroviruses
Volume21
Issue number1
DOIs
Publication statusPublished - Jan 2005

Fingerprint

Indinavir
HIV Protease Inhibitors
Stromal Cells
Ritonavir
Bone Marrow Cells
HIV-1
Stem Cells
Protease Inhibitors
Cell Culture Techniques
Bone Marrow
Cytokines
Stem Cell Factor
Fas Ligand Protein
Growth
HIV Infections
Healthy Volunteers
Fibroblasts
Apoptosis
Membranes
Human immunodeficiency virus 1 p16 protease

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

HIV type 1 protease inhibitors enhance bone marrow progenitor cell activity in normal subjects and in HIV type 1-infected patients. / Isgrò, Antonella; Aiuti, Alessandro; Mezzaroma, Ivano; Ruco, Luigi; Pinti, Marcello; Cossarizza, Andrea; Aiuti, Fernando.

In: AIDS Research and Human Retroviruses, Vol. 21, No. 1, 01.2005, p. 51-57.

Research output: Contribution to journalArticle

Isgrò, Antonella ; Aiuti, Alessandro ; Mezzaroma, Ivano ; Ruco, Luigi ; Pinti, Marcello ; Cossarizza, Andrea ; Aiuti, Fernando. / HIV type 1 protease inhibitors enhance bone marrow progenitor cell activity in normal subjects and in HIV type 1-infected patients. In: AIDS Research and Human Retroviruses. 2005 ; Vol. 21, No. 1. pp. 51-57.
@article{5b33192268b647928730befe716f5a53,
title = "HIV type 1 protease inhibitors enhance bone marrow progenitor cell activity in normal subjects and in HIV type 1-infected patients",
abstract = "HIV-1 protease inhibitors (PIs) may improve hematopoietic functions owing to their direct effects on bone marrow (BM) progenitor cells. In this study we investigated this hypothesis evaluating the effect of adding ritonavir (RTV) and indinavir (IND) on hematopoietic colony formation assays by colony-forming cell (CFC) and long-term culture-initiating cell (LTC-IC) assays, on apoptosis, on cytokine production and stromal cells, in subjects with HIV-1 infection, and in seronegative controls. After PI addition, CFC and LTC-IC assays in HIV-1-infected patients showed levels of colony growth significantly higher than those observed at baseline; the same PI activity on colony formation was observed in healthy subjects. No significant modifications on Fas, the membrane form of Fas (mFas) and Fas-ligand (FasL) expression, and on cytokine production were observed at BM level after the addition of PIs. At baseline, in HIV-1-infected patients, the majority of the stromal cells appeared as large and rounded, whereas after the addition of RTV or IND the stromal cells exhibited a {"}fibroblast-like{"} morphology and produced higher stem cell factor (SCF) and lower MIP-1α levels when compared with the stromal production without the addition of IND. RTV and IND increased colony growth of BM obtained either from HIV-1-infected patients or from normal individuals, in parallel with the normalization of functional and morphological characteristics of stromal cells.",
author = "Antonella Isgr{\`o} and Alessandro Aiuti and Ivano Mezzaroma and Luigi Ruco and Marcello Pinti and Andrea Cossarizza and Fernando Aiuti",
year = "2005",
month = "1",
doi = "10.1089/aid.2005.21.51",
language = "English",
volume = "21",
pages = "51--57",
journal = "AIDS Research and Human Retroviruses",
issn = "0889-2229",
publisher = "Mary Ann Liebert Inc.",
number = "1",

}

TY - JOUR

T1 - HIV type 1 protease inhibitors enhance bone marrow progenitor cell activity in normal subjects and in HIV type 1-infected patients

AU - Isgrò, Antonella

AU - Aiuti, Alessandro

AU - Mezzaroma, Ivano

AU - Ruco, Luigi

AU - Pinti, Marcello

AU - Cossarizza, Andrea

AU - Aiuti, Fernando

PY - 2005/1

Y1 - 2005/1

N2 - HIV-1 protease inhibitors (PIs) may improve hematopoietic functions owing to their direct effects on bone marrow (BM) progenitor cells. In this study we investigated this hypothesis evaluating the effect of adding ritonavir (RTV) and indinavir (IND) on hematopoietic colony formation assays by colony-forming cell (CFC) and long-term culture-initiating cell (LTC-IC) assays, on apoptosis, on cytokine production and stromal cells, in subjects with HIV-1 infection, and in seronegative controls. After PI addition, CFC and LTC-IC assays in HIV-1-infected patients showed levels of colony growth significantly higher than those observed at baseline; the same PI activity on colony formation was observed in healthy subjects. No significant modifications on Fas, the membrane form of Fas (mFas) and Fas-ligand (FasL) expression, and on cytokine production were observed at BM level after the addition of PIs. At baseline, in HIV-1-infected patients, the majority of the stromal cells appeared as large and rounded, whereas after the addition of RTV or IND the stromal cells exhibited a "fibroblast-like" morphology and produced higher stem cell factor (SCF) and lower MIP-1α levels when compared with the stromal production without the addition of IND. RTV and IND increased colony growth of BM obtained either from HIV-1-infected patients or from normal individuals, in parallel with the normalization of functional and morphological characteristics of stromal cells.

AB - HIV-1 protease inhibitors (PIs) may improve hematopoietic functions owing to their direct effects on bone marrow (BM) progenitor cells. In this study we investigated this hypothesis evaluating the effect of adding ritonavir (RTV) and indinavir (IND) on hematopoietic colony formation assays by colony-forming cell (CFC) and long-term culture-initiating cell (LTC-IC) assays, on apoptosis, on cytokine production and stromal cells, in subjects with HIV-1 infection, and in seronegative controls. After PI addition, CFC and LTC-IC assays in HIV-1-infected patients showed levels of colony growth significantly higher than those observed at baseline; the same PI activity on colony formation was observed in healthy subjects. No significant modifications on Fas, the membrane form of Fas (mFas) and Fas-ligand (FasL) expression, and on cytokine production were observed at BM level after the addition of PIs. At baseline, in HIV-1-infected patients, the majority of the stromal cells appeared as large and rounded, whereas after the addition of RTV or IND the stromal cells exhibited a "fibroblast-like" morphology and produced higher stem cell factor (SCF) and lower MIP-1α levels when compared with the stromal production without the addition of IND. RTV and IND increased colony growth of BM obtained either from HIV-1-infected patients or from normal individuals, in parallel with the normalization of functional and morphological characteristics of stromal cells.

UR - http://www.scopus.com/inward/record.url?scp=13144302862&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13144302862&partnerID=8YFLogxK

U2 - 10.1089/aid.2005.21.51

DO - 10.1089/aid.2005.21.51

M3 - Article

VL - 21

SP - 51

EP - 57

JO - AIDS Research and Human Retroviruses

JF - AIDS Research and Human Retroviruses

SN - 0889-2229

IS - 1

ER -