HIV type 1 Tat protein is a survival factor for kaposi's sarcoma and endothelial cells

V. Cantaluppi, L. Biancone, M. Boccellino, S. Doublier, R. Benelli, S. Carlone, A. Albini, G. Camussi

Research output: Contribution to journalArticle

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Abstract

The HIV-1 Tat protein has been directly implicated in the pathogenesis of AIDS-related Kaposi's sarcoma (KS); however, its effects on KS spindle-shaped and endothelial cell apoptosis are largely unexplored. Since susceptibility to apoptosis is relevant for tumor development and response to therapy, we investigated the effects of Tat on KS and endothelial cell survival from apoptosis. The effect of Tat was evaluated in three KS cell lines (KS-imm, KS-C1, and KS-L3) exposed to the chemotherapy agent vincristine, currently used for the treatment of this tumor, and in human umbilical vein-derived endothelial cells (HUVECs) induced to undergo apoptosis by serum withdrawal. Apoptosis was assessed by enzymatic assays, microscopic examination of chromatin and cytoskeleton, evaluation of plasma membrane integrity and subdiploid DNA content, TUNEL assays, and measurement of caspase-3 activity. Tat, in a dose-dependent manner, protected the three KS cell lines and HUVECs from apoptosis induced by vincristine or serum starvation, respectively. This effect appeared to be independent of modulation of Fas, Bcl-2, or Bax expression. In contrast, Tat upregulated Bcl-XL expression and induced a relevant decrease in caspase-3 activity in vincristine-treated KS cells. Taken together, these results suggest that the HIV-1 Tat protein may factor KS development and progression by sustaining endothelial and transformed cell survival.

Original languageEnglish
Pages (from-to)965-976
Number of pages12
JournalAIDS Research and Human Retroviruses
Volume17
Issue number10
DOIs
Publication statusPublished - 2001

Fingerprint

Human Immunodeficiency Virus tat Gene Products
Kaposi's Sarcoma
HIV-1
Endothelial Cells
Apoptosis
Vincristine
Human Umbilical Vein Endothelial Cells
Caspase 3
Cell Survival
Cell Line
In Situ Nick-End Labeling
Enzyme Assays
Starvation
Cytoskeleton
Serum
Chromatin
Neoplasms
Cell Membrane

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Cantaluppi, V., Biancone, L., Boccellino, M., Doublier, S., Benelli, R., Carlone, S., ... Camussi, G. (2001). HIV type 1 Tat protein is a survival factor for kaposi's sarcoma and endothelial cells. AIDS Research and Human Retroviruses, 17(10), 965-976. https://doi.org/10.1089/088922201750290087

HIV type 1 Tat protein is a survival factor for kaposi's sarcoma and endothelial cells. / Cantaluppi, V.; Biancone, L.; Boccellino, M.; Doublier, S.; Benelli, R.; Carlone, S.; Albini, A.; Camussi, G.

In: AIDS Research and Human Retroviruses, Vol. 17, No. 10, 2001, p. 965-976.

Research output: Contribution to journalArticle

Cantaluppi, V. ; Biancone, L. ; Boccellino, M. ; Doublier, S. ; Benelli, R. ; Carlone, S. ; Albini, A. ; Camussi, G. / HIV type 1 Tat protein is a survival factor for kaposi's sarcoma and endothelial cells. In: AIDS Research and Human Retroviruses. 2001 ; Vol. 17, No. 10. pp. 965-976.
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